Gene Synthesis

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Overview
Gene synthesis is a sophisticated biotechnological process that involves the artificial construction of custom DNA sequences based on specified genetic information. Gene synthesis plays a crucial role in various fields such as genetic engineering, synthetic biology, drug development, and protein expression studies.

Tsingke is a premier gene synthesis service provider, offering high-quality services with fast turnaround time. We have established the autonomous research and development, automated self-aggregation TSINGKE Gene Factory synthesis platform, equipped with advanced Tsynth™ synthesis technology. Based on TSINGKE HELIXTECH, we integrate synthetic materials, synthetic equipment, and synthesis processes in the gene synthesis elements, achieving a highly intelligent production system. Continuous research and upgrades to our synthesizers and materials ensure the production of highly accurate oligos, enabling stable and efficient gene synthesis with timely delivery. For quality assurance, all Tsingke-constructed plasmids undergo rigorous NGS and Sanger sequencing validation, ensuring 100% sequence accuracy.
Advantages
Achieved construction of 200kb plasmid
Achieved construction of 200kb plasmid
Proficient in synthesizing large sequences
Optional
Optional
Choose from 160+ commercial vectors at no extra cost, and enjoy free codon optimization
Fast Turnaround Time
Fast Turnaround Time
As fast as 5 days for standard gene synthesis
Unique Intelligent Splitting Algorithm
Unique Intelligent Splitting Algorithm
Rapidly and scientifically designs oligos for each gene
Service Type

Tsingke offers a comprehensive suite of gene synthesis services to cater to diverse research needs, such as Standard Gene Synthesis, ssDNA, etc. Tsingke leverages cutting-edge technologies and a highly intelligent production system to ensure accuracy, efficiency, and timely delivery. Also, tsingke is committed to delivering reliable and efficient gene synthesis solutions to researchers and biotechnologists worldwide. Contact us now for more details or to get started on your project.

Service Details
Standard Gene Synthesis & ProLongGene
- Highly Customized: From simple sequences to complex sequences
- Gene Length: Up to 200 kb
- Standard Deliver 1~4 μg plasmid
- Fast Turnaround: As fast as 5 business days
DNA Fragment
- From 100 bp to 1.2 kb
- Deliver 500 ng or 1 μg PCR products
- Efficient turnaround time of 2 to 5 business days
Single-stranded DNA (ssDNA)

- From 100 nt to 6000 nt
- Deliver lyophilized product powde
Plasmid Preparation
- Research grade
- Industrial grade
- Endotoxin <0.1 Eu/μg, <0.01 Eu/μg, <0.005 Eu/μg
PCR Cloning & Subcloning 
-100% Sequence Accuracy: Guaranteed with Sanger sequencing and NGS
-Not limited by restriction enzyme cutting sites
-Customizable: Clone target gene fragment at any site in any vector system
Mutagenesis
-100% Sequence Accuracy: Guaranteed with Sanger sequencing and NGS
-Unlimited Sites: Introduce your mutations at any site
-Cost-effective: All mutations found within a 30-base region will be defined as one whole mutation
Facility
Facility
Facility
Facility
Facility
Related Resource
FAQ
Does Tsingke provide codon optimization? Does optimization have an impact on gene expression?

Yes, Tsingke offers free codon optimization.

Codon optimization enhances gene expression by using preferred codons and avoiding rare ones for a specified host. This optimization correlates strongly with improved gene expression levels. Tsingke's unique GeneOptimizer software supports optimization for tens of thousands of hosts and offers customized services. Key optimization parameters include codon preference, GC content, restriction enzyme sites (deletion), poly structures, and sequence repeats.

Can you keep my sequence information confidential?

Yes, all customer information will be kept confidential.

Can I send my vectors to you? Are there any requirements for my provided vector?

Yes, you can use your own vector. It is recommended to ship your vector in advance; otherwise, the turnaround time may be extended by 3 business days to verify the vector.

We need you to ship at least 2 μg of the plasmid and provide the full sequence information of the vector. If the full sequence is not available, please provide at least 200 bp upstream and downstream of the cloning site, along with the vector's antibiotic resistance information.

What vector does Tsingke use by default?

By default, we provide pUC57 vectors containing Amp (ampicillin) for free. We also offer a library of over 160 vector options available for free.

What information do I need to prepare a quote?

We need the insert DNA, host species (if codon optimization is needed), and restriction sites at the 5'/3' ends. Specify any internal modifications to avoid and additional elements like the Kozak sequence or stop codon.

How do I place an order?

Complete the gene synthesis Request Forms and email it to info@tsingke.com.cn. For special inquiries or project requirements, please specify them in the email. Our technical support team will promptly provide a detailed quotation.

*For Research Use Only. Not for use in diagnostic procedures.
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