PCR cloning amplifies specific DNA fragments without restriction sites, while subcloning transfers DNA between vectors—sometimes referred to as subcloning vectors. Our cloning and subcloning techniques support gene cloning, sequencing, and functional studies.
Tsingke offer professional subcloning service and services of cloning PCR products, utilizing advanced technologies such as seamless cloning to efficiently and accurately insert your amplified fragments into specified vectors.
| Length | Turnaround time (Calendar Day) | Vector | Deliverables |
| < 1.5 kb | 7-10 | Customer-specific | (1) 1 tube of lyophilized plasmid DNA (about 1~4 μg/tube); (2) QC files: Sequencing map (.abl file); Target sequence (.seq file); COA Report(electronic). |
| 1.5kb~3kb | 7-11 | ||
| 3kb~6kb | 10-15 | ||
| 6kb~8kb | 13-18 | ||
| ＞8kb | Evaluation |
Download the order form right and email it to info@tsingke.com.cn, or "Send Your Request" to submit your inquiry online.
a. Templates: For cloning services, you should provide and send us the template plasmid(≥2μg). If the plasmid was constructed by Tsingke and the order was placed within the last year, you do not need to provide it again.
b. Vector details: Choose from our 200+ free gene cloning PCR vectors or provide your PCR cloning vector.
c. Provide the sequences and resistance information for both template and vector, along with the target sequence and cloning siteinformation.
d. Order Form: Download the "Tsingke Gene Synthesis Order Form" from our website, fill in the relevant fields according to your needs,and send it via email to info@tsingke.com.cn to place your order.
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