Name: PCR Cloning & Subcloning
Brand: Tsingke Biotech
SKU: 00285
Availability: InStock
Rating: 5 (1 reviews)

Overview

PCR (Polymerase Chain Reaction) cloning and subcloning are essential techniques commonly used in molecular biology for amplifying and manipulating DNA fragments.

PCR cloning streamlines the process by using the polymerase chain reaction to amplify a specific DNA fragment of interest from a template DNA, eliminating the need for specific restriction enzyme sites as in traditional methods. Primers are designed based on the sequence of the target gene, enabling direct amplification of the desired DNA fragment.

Subcloning involves transferring a DNA fragment from one vector to another.

PCR cloning and subcloning enable researchers to clone and manipulate DNA fragments efficiently for various applications in gene expression, sequencing, protein production, and functional analysis.

Tsingke offers a one-stop service ranging from gene synthesis and vector construction to PCR cloning and subcloning. With advanced technology and extensive expertise, we ensure high-quality cloning services to support your research needs.

Advantages

Accuracy

Guaranteed with Sanger sequencing and NGS

No limit

Not limited by restriction enzyme cutting sites

Clonable

Clone target gene fragment at any site in any vector system

Service Details

Length	*Turnaround time (Business Day)	Deliverables
<3 kb	7~10	1 tube of lyophilized plasmid DNA (about 1~4 μg/ tube); Sequencing map (.abl file); Target sequence (.seq file); COA Report(electronic).
3 kb~5 kb	10~12
5 kb~7 kb	12~15
>7 kb	Evaluation

*Note: Get your accurate estimated turnaround time by emailing gene@tsingke.com.cn

Workflow

oligo design & synthesis

Order Design

oligo design & synthesis

PCR amplification or digestion

Sequence Acquisition

PCR amplification or digestion

linearized the purified DNA fragment with the cut destination vector

Vector Construction

linearized the purified DNA fragment with the cut destination vector

QC for NGS & Sanger sequencing

Quality Control

QC for NGS & Sanger sequencing

ship the plasmid containing your target gene fragment

Delivery

ship the plasmid containing your target gene fragment

Related Resource

Tsingke_Gene Synthesis_Order Form

Tsingke_Gene Synthesis_Flyer-V1.1.1.240129

Tsingke_Gene Synthesis_Brochure_V1.1.1

FAQ

What do I need to provide for PCR cloning services?

a.Templates： Provide and send us the template plasmid. If the plasmid was constructed by Tsingke and the order was placed within the last year, you do not need to provide it again.
b.Vector details: Choose from our 160+ free vectors or provide your vector.
c.Provide the sequences and resistance information for both template and vector, along with the target sequence and cloning site information.
d.Order Form: Download the "Tsingke Gene Synthesis Order Form" from our website, fill in the relevant fields according to your needs, and send it via email to gene@tsingke.com.cn to place your order. the a.Templates： Provide and send us the template plasmid. If the plasmid was constructed by Tsingke and the order was placed within the last year, you do not need to provide it again.b.Vector details: Choose from our 160+ free vectors or provide your vector.c.Provide the sequences and resistance information for both template and vector, along with the target sequence and cloning site information.d.Order Form: Download the "Tsingke Gene Synthesis Order Form" from our website, fill in the relevant fields according to your needs, and send it via email to gene@tsingke.com.cn to place your order.

Can I provide PCR products directly for PCR cloning?

No, the template sequence needs to be first constructed into a T-vector or another vector. Then, amplify using the plasmid as the template. Alternatively, you can choose our gene synthesis service by simply providing the DNA sequence.