Custom RNA Oligos

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Overview

RNA oligos, short for ribonucleic acid oligonucleotides, are single-stranded synthetic RNA sequences that can serve as an important regulator of gene function analysis and development of novel therapeutic strategies. With precise customization options, researchers can design RNA oligos with specific sequences to target and manipulate gene expression, study RNA structure and function, or develop therapeutic interventions for diseases. Additionally, RNA oligos can be chemically modified to enhance stability, specificity, or delivery efficiency in experimental settings.

Tsingke provides high-quality and cost-effective custom RNA oligos with various synthesis types and specific modifications to meet researchers' different needs, facilitating advancements in gene editing, germplasm transformation, genetic regulation, etc. To ensure product qualification, all RNA oligos use high-quality raw materials and synthesis processes, are purified by OPC or HPLC methods, and are 100% mass spectrometrically detected. We address challenges such as low coupling efficiency between bases, product decomposition susceptibility, demanding operating environments, and high costs, providing reliable solutions for your research needs.

Advantages
Various Product Types
Various RNA synthesis products, such as sgRNA, siRNA, miRNA, etc.
Rapid Synthesis Cycle
Regular orders can be completed in 3-4 days
Various QC Methods
100% mass spectrometry with a variety of additional detection options
Service Details

Name

Type

Quantity

Purification

Turnaround Time
(Bussiness Day)

QC

Modification

sgRNA

sgRNA

1.5 nmol

OPC

7

Mass Spectrometry

Add specific modifications 

1.5 nmol

HPLC 

12~15

Mass Spectrometry

HPLC

crRNA

crRNA

5 nmol /2 OD

HPLC 

12~15

Mass Spectrometry

HPLC

siRNA
Oligo Duplex

1 siRNA 

5 nmol /2 OD

HPLC

3~4

Mass Spectrometry

HPLC

/

siRNA NC

2.5 nmol /1 OD

Available in stock

/

3 siRNA,
1 Guaranteed

5 nmol /2 OD

3~4

3 unique siRNA duplexes/
FAM labeled NC (1 nmol)/
Negative and Positive control (2.5 nmol each)
At least one guaranteed gene knockdown (≥70%)

4 siRNA,
1 Guaranteed

5 nmol /2 OD

3~4

4 unique siRNA duplexes/
1 FAM labeled NC (1 nmol)/
Negative and Positive control (2.6 nmol each)
At least one guaranteed gene knockdown (≥70%)

miRNA

miRNA mimics

5 nmol/2 OD

HPLC

3~4

Mass Spectrometry

HPLC

/

miRNA inhibitor

10 nmol/2 OD

mimics/
inhibitor(NC)

1 OD

Available in stock

miRNA agomir

2 OD

5~7

miRNA antagomir

2 OD

agomir/
antagomir (NC)

2 OD

ASO

PS-ASO

2 OD

HPLC

3~4

Mass Spectrometry

HPLC

/

LNA-ASO

2 OD

OMe-ASO

2 OD

Workflow
High-throughput synthesizer from pmol to mmol levels
Synthesis
High-throughput synthesizer from pmol to mmol levels
Waters 2695 & Waters 2767 automatic purification and HPLC purification
Purification
Waters 2695 & Waters 2767 automatic purification and HPLC purification
Mass quality inspection, HPLC and other additional quality inspections
QC
Mass quality inspection, HPLC and other additional quality inspections
Automatic dispensing instrument for accurate dispensing
Distribution
Automatic dispensing instrument for accurate dispensing
Tube or Customized、Lyophilized RNA、COA Report
Delivery
Tube or Customized、Lyophilized RNA、COA Report
Case
Figure 1: Molecular weight deviation ≤ 0.05%
             
Figure 2: HPLC: Purity = 97.29%
Related Resource
FAQ
What is the maximum length and the types of RNA oligos synthesized by Tsingke?
We specialize in synthesizing RNA oligos up to 180 nucleotides in length, and the types mainly include siRNA, sgRNA, miRNA, ASO, and various modified RNAs.
What are the maximum absorption and emission wavelengths of commonly used RNA fluorescent dyes?
FAM has a maximum absorption at 495 nm and maximum emission at 520 nm. Cy3 has a maximum absorption at 550 nm and maximum emission at 565 nm. Cy5 has a maximum absorption at 643 nm and maximum emission at 667 nm.
How should siRNA be dissolved and stored?
Before opening, centrifuge the tube. Add sterile RNase-free H2O or ddH2O to prepare a 20 μM stock solution. Aliquot as needed and store at -20°C to -80°C.
Why is a positive control important in RNA interference experiments?
A positive control is crucial for validating the experimental system. When the expected results from the siRNA positive control are observed, it ensures that the transfection, RNA extraction, and QC methods used are reliable.
What roles do positive and negative controls play in RNAi experiments, and how should I select?
Positive controls are verified siRNAs targeting housekeeping or reporter genes, used to assess the feasibility of the experimental methods. Tsingke provides effective siRNA positive controls targeting GAPDH, ACTB, GFP/EGFP. 
Negative controls are non-specific siRNAs used to demonstrate the specificity of siRNA effects and can be selected from universal or scrambled sequences based on requirements.
Should the results of negative controls be the same across different groups? What if there is significant deviation?
Normally, the results of negative controls should be similar across different groups. Significant deviations may indicate issues with the accuracy of experimental results. 
For certain genes, particularly those related to cellular stress or immune response, varying responses to external stress might cause inconsistent gene expression across control groups.
What should I do if the silencing effect is not satisfactory?
The two most common reasons for unsatisfactory silencing effects are low transfection efficiency and suboptimal siRNA sequence design.
1.Check Transfection Efficiency: If you are using siRNA for the first time or working with a new cell line, we recommend testing and optimizing the transfection efficiency. If you have already optimized the transfection conditions and the problem persists, consider switching to a different transfection reagent or method, as this might improve transfection efficiency.
2.Evaluate siRNA Sequence Design: If the transfection efficiency has been improved but the silencing effect is still not satisfactory, it may be due to ineffective siRNA sequence design.
**For Research Use Only. Not for use in diagnostic procedures.
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