Relative mRNA expression was normalized to β-actin using the 2−ΔΔCt method. Primers were synthesized by Beijing Qingke and used as follows: Mouse α-SMA (ACTA2) Forward (F): 5'-GCCCCTGAAGAGCATCCGAC-3'; ACTA2 Reverse (R): 5'-CCAGAGTCCAGCACAATACCAGT-3', Mouse collagen I (COL1A1) F: 5'-CTGGTGCTCG CGGTAACGAT-3'; COL1A1 R: 5'-CAGCACCAGGGTTTCCAGCA-3', Mouse fibronectin (FN1) F: 5'-GATGTCCGAACAGCTATTTACCA-3'; FN1 R: 5'-CCTTGCGACTTCAGCCACT-3', Mouse SIRT1 F: 5'-TTCTATACCCCATGAAGTGCCTC-3'; SIRT1 R: 5'-CACCACCTAGCCTATGACACA-3', Mouse SLC7A11 F: 5'-CATACTCCAGAACACGGGCAG-3'; SLC7A11 R: 5'-AACAAAAGCCAGCAAAGGACCA-3', Mouse p53 (TP53) F: TCCTCCCCAGCATCTTATCCG; TP53 R: CCATGCAGGAGCTATTACACA, and Mouse β-actin F: 5'-ACATCCGTAAAGACCTCTATGCC-3'; β-actin R: 5'-TACTCCTGCTTGCTGATCCAC-3'.
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