Total RNA was extracted using TRIzol reagent. Reverse transcription was performed with PrimeScript RT Master Mix. Gene-specific primers (Table 1) designed by Primer Premier 6.0 were synthesized by Tsingke Biotechnology, SYBR® Premix EX TaqTM Fluorescent real-time Quantification Kit from Takara was used. Relative mRNA expression was calculated by 2 − ΔΔCt method using β-actin as endogenous control. Statistical comparisons were performed using one-way ANOVA in SPSS.
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