Abstract
Complete Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Cat No. 11965092, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific, Cat No. A5256701) and 1% penicillin-streptomycin (Beyotime, Cat No. C0222, Shanghai, China) was used for culturing all cell lines. Beijing Tsingke Biotech (Beijing, China) designed and manufactured short interfering RNAs (siRNA). Before usage, the siRNA powder was dissolved in RNase-free water.
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