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NLR Risk Score for Predicting Patient Prognosis in Hepatocellular Carcinoma and Identification of Oncogenic Role of NLRP5 in Hepatocellular Carcinoma

Abstract

Complete Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Cat No. 11965092, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific, Cat No. A5256701) and 1% penicillin-streptomycin (Beyotime, Cat No. C0222, Shanghai, China) was used for culturing all cell lines. Beijing Tsingke Biotech (Beijing, China) designed and manufactured short interfering RNAs (siRNA). Before usage, the siRNA powder was dissolved in RNase-free water.


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