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Malignant epithelia cells-derived spermine induces APOE+ macrophages to suppress tumor immunity in adenocarcinoma of the esophagogastric junction

Abstract

2.17 siRNA transfection siRNAs targeting human SMS, mouse sms and negative control (siNC) were designed and synthesized by Tsingke (Beijing, China). The siRNAs were transfected into MFC cells and OE-19 cells using Lipofectamine 2000 (Thermofisher) according to the manufacturer’s instructions.


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