The open reading frame (ORF) sequence of SeXTH23 was downloaded from the transcriptome database of S. europaea (unigene ID: i1_HQ_samplee11669_c284462_f3p3_1081) (Tiika et al. 2021). The cDNA fragment containing the ORF of SeXTH23 was amplified with primers SeXTH23-CDS (Table S1), and was cloned into the PMD 18-T vector (6011, Takara Biotech Co., Ltd., Dalian, China) using the T4 DNA ligase (2011A, Takara Biotech Co., Ltd., Dalian, China), and sequenced by TsingKe Biological Technology Co., Ltd. (Xi’an, China).|||The ubiquitin-conjugating (UBC) gene (Unigene40024_All) was used as an internal control gene (Xiao et al. 2014). The specific SeXTH23-RT-qPCR primers were designed and synthesized by TsingKe Biological Technology Co., Ltd. (Xi’an, China), which were tested for amplification efficiency and specificity (Table S1). All the treatments had 3 replicates and 3 technical repeats.
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