The sense sequence of the NC mimic is: 5'-UUUGUACUACACAAAAGUACUG-3', and the antisense sequence is: 5'-AAACAUGAUGUGUUUUCAUGAC-3'. The reporter plasmids used in the experiment were the pmirGLO-hsa-circ-0122913-WT and pmirGLO-hsa-circ-0122913-MUT plasmids, synthesized by Beijing Tsingke Biotech Co., Ltd. For the transfection experiments, 293T cells, which were obtained from Chinese National Collection of Authenticated Cell Cultures, at ~80% confluency in 24-well plates were co-transfected with 20 pmol of miRNA mimics and 500 ng of reporter plasmids using Lipofectamine 3000 transfection reagent (cat. no. L3000150; Thermo Fisher Scientific, Inc.).
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