(2,3-dioleoyloxypropyl)-trimethylammonium (DOTAP) was purchased from Corden Pharma (Liestal, Switzerland). The primers of real-time fluorescent quantitative PCR (qRT-PCR) were synthesized by Tsingke Biotechnology Ltd. (Beijing, China), and are as follows: CXCL9 (forward: 5’-TGAAGAAGCTGATGAAAGAATGGGA-3’, reverse: 5’TCCTCTTCTGAGATGAGTTTTTGTTCT-3’), αPD-1 scFv (forward: 5’-AACAG AGTTACAGTACCCCGTGGA-3’, reverse: 5’-TCCTCTTCTGAGATGAGTTTTTG TTCT-3’) and GAPDH (forward: 5’-AGGTCGGTGTGAACGGATTTG-3’, reverse: 5’-TGTAGACCATGTAGTTGAGGTCA-3’). Dulbecco’s Modified Eagle Medium (DMEM) was purchased from Gibco (CA, USA), and fetal bovine serum (FBS) was from Invitrogen.
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