Custom DNA Oligo

According to the purpose of the experiment. In general, about 20 bases of primer 2 OD in PCR amplification can do 400 rxns standard PCR reactions of 50 μL system. If it is to do gene splicing or annealing to do the ligation, 1 OD is enough.

When combining long chain primers, more reagents are required, especially primers longer than 90 base. Due to the increase in cost, the price is higher.

The entire process from synthesis, QC to packaging can be highly customized. Customizable options include, but are not limited to:

(1) Packaging in 1.5 mL, 15 mL, or 50 mL tubes;

(2) Capillary electrophoresis analysis;

(3) High-performance liquid chromatography (HPLC) analysis;

(4) Polyacrylamide gel electrophoresis (PAGE) analysis;

(5) Quantitative PCR (qPCR) analysis;

(6) Full-wavelength scanning;

(7) OD260/280 measurement;

(8) Provision of 1 OD sample with each shipment;

(9) Specialized consumables;

(10) Customized Certificate of Analysis (COA).

Synthetic primers may be yellowish brown, white or transparent, which is related to the base composition of the primers and the preparation process of synthesis, primers with high A and G content in the sequence and large OD value are usually yellowish brown, so the yellowish brown primers will not have any effect on the experiment.

The common purification methods are DSL, OPC, PAGE, HPLC and Dual PAGE & HPLC.

It will not degrade, and the dry primer can be stably stored at room temperature for at least two weeks. The average transport time is usually 5days, so the primers you receive will not degrade.