Total RNA was isolated via RNAiso Plus reagent (TaKaRa, Japan) following the manufacturer’s instructions. Complementary DNA (cDNA) was synthesized via a reverse transcription kit (Takara, Dalian, China). Quantitative real-time PCR was performed to determine the mRNA levels via the SYBR Premix Ex Taq II Kit (TaKaRa, Japan) and primers synthesized by Tsingke (China). The experiments were conducted in triplicate.
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