Overexpression and the RNA interference assays The over-expression plasmids PCDNA3.1-DNMT1, PCDNA3.1-TET2 and PCDNA3.1 empty vector were purchased from Tsingke Biotechnology Co.,Ltd. Goose embryonic myoblasts were transfected using Lipofectamine 3000 (thermofisher, USA) according to the manufacturer’s protocol.|||DNMT1 siRNAs, TET2 siRNAs and a non-targeting siRNA were purchased from Tsingke Biotechnology Co.,Ltd. Goose embryonic myoblasts were transfected with siRNAs using Lipofectamine 3000 according to the manufacturer’s protocol.
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