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Efficacy of Whole-Cell-Based Mono- and Bi-Valent Vaccines Against Nocardia seriolae and Aeromonas veronii in Largemouth Bass, Micropterus salmoides

Abstract

The relative expression of target genes was analyzed using the 2−ΔΔCT method [37], with β-actin serving as the housekeeping gene based on previous studies [38,39,40]. All primers were provided by Tsingke (Wuhan, China), and their sequences are listed in Table 1.


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