The osteoblasts (MC3T3-E1 cells) were cultured with the mix of γδ T cell supernatant and osteogenic induction medium. Effective Plxnb1, Plxnb2 siRNA and control siRNA (Tsingke Biotech) were obtained from the supplier. When the cells reached 50% confluence, they were transfected with Plxnb1, Plxnb2 siRNA using TSnanofect V2 Transfection Reagent (Tsingke Biotech) according to the manufacturer's protocol. The siRNA and qPCR primers used are listed in Table S1.
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