GAPDH was chosen as an internal reference, and the expression of each gene was normalized using the 2−ΔΔCT method. Synthesis of all primers was carried out by Tsingke Biotech (Shanghai, China). Details of all primers used in this study are provided in Supplementary Table S3.|||Lentiviral transfection to construct stable cell lines The pLKO.1-shSAA1-Puro plasmid for shRNA-mediated knockdown of human SAA1 was designed and synthesized by Tsingke Biotech (Shanghai, China). The PGMLV-shSAA1-Puro plasmid for knockdown of murine SAA1 was designed and synthesized by Geneseed (Shanghai, China). The sgRNA sequence for CRISPR/Cas9-mediated knockout of murine SAA1 was designed by Zhang Lab and synthesized by Tsingke Biotech (Shanghai, China), and subsequently cloned into the lentiCRISPR V2-sgSAA1-Puro vector. In addition, a full-length cDNA sequence of human SAA1 was constructed by You Bao (Hunan, China) for overexpression in ovarian cancer cells.
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