Small interference RNA (siRNA) interference The fxr, sumo1 and pias1 siRNA was obtained from Tsingke Biotech (Beijing, China), GenePharma Biotech (Shanghai, China) and Tsingke Biotech, respectively (Supplemental Table 3). We used the reagent Entranster™-R4000 Reagent kit (4000-4, Engreen Biosystem) for transient transfection.
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