The S. cerevisiae strains used in this study are listed in (Table 2). All primers were synthesized by Beijing Tsingke Biotechnology Co., Ltd. (Beijing, China) and are listed in (Table S1). The plasmids used in this study are listed in (Table S2).|||The gene At4CL1 was PCR-amplified from Arabidopsis thaliana cDNA, while EcaroL was amplified from E. coli genomic DNA. Codon optimization and gene synthesis for all other heterologous genes employed in this study were performed by Beijing Tsingke Biotechnology Co., Ltd. (Beijing, China) listed in (Table S3). The mutant genes ARO3D154N, ARO4K229L, ARO7G141S, and PYK1D146N were generated via overlap extension PCR [26].
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