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WGCNA and LASSO regression-based selection and validation of microRNA biomarkers of β-thalassemia

Abstract

Levels of mRNA and miRNA expression were normalized to GAPDH and U6, respectively. Primers used for this study are presented in Supplemental Table S2, and were synthesized by Tsingke Biotechnology (Shanghai, China). The 2−ΔΔCt method was used to compute relative expression.


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