Although the GenBank submissions lack protein-coding genes, preventing their use in phylogenetic tree construction and comparative analysis with known species, we have provided the protein-coding sequences to facilitate future research. PCR amplification and purification were carried out in a 25 μL total volume containing 12.5 μL of 2 × Hieff Canace Plus PCR Master Mix (Cat. No. 10154ES03, Yeasen Biotechnology, Shanghai, China), 1 μL each of forward and reverse primers (TsingKe, Qingdao, China), 1 μL of template genomic DNA, and double distilled water to adjust the final volume. Amplified products were electrophoresed on a 1% agarose gel stained with Safe Red (Cat. Nos. RM02852 and RM19009, ABclonal Biotechnology Co., Ltd., Wuhan, China) and visualized under UV light (Ai et al. 2025).
Stay updated with the latest industry news and expert insights.
Subscribe now to receive:
● Industry updates
● Exclusive expert insights and analysis
Enter your email to stay ahead!
