LILRB4 knockdown was achieved through lentiviral transduction. LILRB4-targeting shRNA sequences (Beijing Tsingke) were cloned into pLKO.1-puro lentiviral vectors and lentiviral particles were produced by co-transfecting HEK293T cells with shRNA plasmids and packaging plasmids (psPAX2 and pMD2.G) using Lipofectamine 3000.
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