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Engineered Fusion Enzyme-Mediated Non-Consecutive Cyclization-Glycosylation Enables Heterologous Synthesis of Antifungal Enfumafungin

Abstract

General Materials and Experimental Procedures Synthesis of genes and primers were performed by Sangon Biotech Co., Ltd. (Shanghai, China) or Tsingke Biotech Co., Ltd. (Beijing, China). PCR was conducted using an A100 thermal cycler (LongGene, China) and 2×Phanta Flash Master Mix (Vazyme, China).


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