This genomic DNA served as the template for PCR identification, which included detecting the expression sequence tag (EST) of eGFP, the EST of the reference gene PTB2 in stylo, the EST of the rolB gene from the A. rhizogenes Ri plasmid, and the EST of the hygromycin B phosphotransferase (Hyg) gene present in the pCXSN plasmid. All primers used for PCR identification were synthesized by Tsingke Biological Technology Co., Ltd. (Beijing, China), and their sequence information is provided in Table S1. The reaction products were analyzed by electrophoresis on a 1.5% (w/v) agarose gel, stained with ethidium bromide, and visualized under ultraviolet light (Fig. S1B).
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