Three microliters of each PCR product were run on 1% (w/v) agarose gels and stained with ethidium bromide [10]. The PCR products were purified and subjected to bidirectional sanger sequencing using the amplification primers at TsingKe Biological Technology (Kunming, China) ITS1F and ITS4. Sequences were edited manually using Sequencher™ 4.1.4 (Gene Codes Corporation, Ann Arbor, MI, USA).
Stay updated with the latest industry news and expert insights.
Subscribe now to receive:
● Industry updates
● Exclusive expert insights and analysis
Enter your email to stay ahead!
