2.5 Small-interfering RNA (siRNA) transfection RNA oligonucleotides targeting IL-27 (palindromic sequence: CAAUCAGGUGUCAUCCCAA) and PGC-1α (palindromic sequence: GUAGCGACCAAUCGGAAAUTT) were procured from TSINGKE (Beijing, China) and GenePharma (Shanghai, China) to facilitate the silencing of the target proteins. Negative control siRNAs, which exhibited no homology with the mouse genome, were also obtained from TSINGKE and GenePharma. The 3T3-L1 cells were transfected with siRNA utilizing Lipofectamine 2000 (Invitrogen, USA).
Stay updated with the latest industry news and expert insights.
Subscribe now to receive:
● Industry updates
● Exclusive expert insights and analysis
Enter your email to stay ahead!
