Eukaryotic expression plasmid pCAGGS-MFN2-Myc, pCAGGS-MAVS-V5, pCAGGS-MAVS-Flag, pCAGGS-UL38-Flag, pCAGGS-UL11-Flag, pCAGGS-UL16-Flag, pCAGGS-UL19-Flag, pCAGGS-RIG-I-Flag, pCAGGS-Ub-HA, pCAGGS-K6R-Ub-HA, pCAGGS-K11R-Ub-HA, pCAGGS-K27R-Ub-HA, pCAGGS-K29R-Ub-HA, pCAGGS-K33R-Ub-HA, pCAGGS-K48R-Ub-HA, pCAGGS-K63R-Ub-HA, Mito-DsRed, dual luciferase reporter plasmids pGL4-IFN-β-Luc and pRL-TK were all preserved by the Poultry Disease Control Research Center of Sichuan Agricultural University. All the primers used for plasmids construction were synthesized by Tsingke Biotechnology. All constructed plasmids were analyzed and verified by DNA sequencing.|||Primers used for Q-PCR are seen in Table 1. All the primers used for Q-PCR were synthesized by Tsingke Biotechnology or labeled by TaKaRa Biotechnology (Dalian, China).
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