The PrimeScript™ RT Reagent Kit (TAKARA, RR037A) was adopted for reversely transcribing one microgram RNA sample into cDNA through a 15-min incubation at 37°C, with the reaction being terminated by a 5-s heating step at 85°C. Afterwards, 2 µL of the cDNA and 0.5 µM primer pairs (synthesized by Beijing Qingke) were assembled with the YEASEN Hieff™ qPCR SYBR Green Master Mix (No Rox) (Cat No. 11201ES08) into a reaction system as specified by the manufacturer. Thermocycling initiated with a 3-min denaturation step at 95°C.
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