Candidate indels were selected for marker development based on their genomic location and observed insertion/deletion patterns, with confirmation by visualization in IGV [21]. PCR primers were designed using Primer Premier 5 and synthesized by Beijing Tsingke Biotech Co., Ltd. (Beijing, China) (https://www.bioprocessonline.com/doc/primer-premier-5-design-program-0001, accessed on 1 September 2025). Each PCR was performed in a 10 μL reaction volume containing 5 μL 2× PCR Mix, 0.4 μL of each primer, 0.5 μL template DNA, and nuclease-free water to volume.
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