We constructed a tobacco rattle virus 2 (TRV2) PomiR164a vector using the short tandem target mimic (STTM) method to obtain a silencing vector for PomiR164a. The STTM PomiR164a sequence was synthesized by Beijing Qingke Biotechnology Co., Ltd. and connected to vector TRV2. Suitable restriction enzyme sites were selected, and Primer Premier 5.0 software was used to design primers to construct the silencing expression vector of PoLBD25.
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