Tsingke has a complete industrial chain in nucleic acid synthesis specializing in the development & manufacturing of genomics and biosynthesis products. Tsingke's wide-ranging expertise and specialized platforms offering total solutions from raw materials and equipment to synthetic products and services.
Achieved construction of up to 200 kb plasmids and proficient in synthesizing large or complex sequences.
Rich selection of gene products, including custom synthesis and choice from 200+ commercial vectors with free codon optimization.
Turnaround as fast as 4 calendar days with ≥99.5% delivery success rate, backed by Sanger sequencingand NGS double verification.
Professional sequence and codon optimization enhance protein expression efficiency; mature solutions for difficult or toxic genes and long/complex fragments
| Type | Introduction |
| Standard Gene Synthesis | Highly Customized: From simple sequences to complex sequences Gene Length: Up to 10 kb Standard delivery 1~4 μg plasmid Fast Turnaround: As fast as 4days |
| ProLong Gene Synthesis | Up to 200 kb Standard delivery 1~4 μg plasmid One-step assembly of DNA fragments |
| Custom shRNA | Designs and synthesizes highly specific shRNA sequences Offers efficiency-guaranteed packages Provides ready-to-use, endotoxin-free plasmids for direct transfection |
| Gene Fragments | From 100 bp to 1.2 kb Deliver 500 ng or 1 μg PCR products Efficient turnaround time of 2 to 3 days |
Sequence Design & Optimization: sequence analysis & codon optimization or shRNA design.
Sequence Design & Optimization: sequence analysis & codon optimization or shRNA design.
Oligo Synthesis: design & synthesis.
Oligo Synthesis: design & synthesis.
Construction: overlap PCR & gene assembly.
Construction: overlap PCR & gene assembly.
Quality Control: QC for NGS & Sanger sequencing.
Quality Control: QC for NGS & Sanger sequencing.
Delivery: ship the plasmid containing your gene/shRNA insert.
Delivery: ship the plasmid containing your gene/shRNA insert.
Building genetic tools to decipher life's mechanisms.
Developing next-generation precision therapies.
Accelerating macromolecular drug innovation.
Creating detection and preventive products.
Yes, Tsingke offers free codon optimization.
Codon optimization enhances gene expression by using preferred codons and avoiding rare ones for a specified host. This optimization correlates strongly with improved gene expression levels. Tsingke’s unique Geneoptimizer software supports optimization for tens of thousands of hosts and offers customized services. Key optimization parameters include codonpreference. GC content, restriction enzyme sites (deletion), poly structures, and sequence repeats.
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