Griess reagent for nitrite oxide (NO), the primary antibody dilution buffer and secondary antibody dilution buffer were obtained from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Primers used to amplify iNOS, IL-6, IL-10, TNF-α and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were synthesized at Tsingke Biotechnology Co., Ltd. (Beijing, China). Polyclonal antibodies p65, IκB, phosphorylated protein65 (p-p65), phosphorylated inhibitor of nuclear factor kappa-B (p-IκB) and β-actin were purchased from Proteintech Group, Inc (Wuhan, China).
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