sgRNA

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Overview

Small guide RNA(sgRNA) is a crucial component of the CRISPR gene knockout and knock-in system. Initially discovered guide RNA consists of two parts: tracRNA and crRNA. When these two parts are fused and expressed together, the resulting sgRNA can effectively perform the guide function by combining with the Cas9 protein and directing the Cas9 enzyme to target and cut genomic DNA.

 

Commonly used sgRNA is transfected into cells in plasmid form. It can be designed either on the same plasmid with the Cas protein or on separate plasmids. However, unless packaged into a virus, either method results in low editing efficiency due to the large plasmid fragments, and there is a higher risk of off-target effects and carcinogenicity.

 

These issues are completely avoided by using sgRNA oligos. Chemically synthesized sgRNA can form ribonucleoprotein complexes (RNPs) with Cas9 protein in vitro. Compared to other delivery methods, RNPs exhibit higher editing efficiency, lower off-target effects, and minimal immunogenicity.

Advantages
Low Cytotoxicity
 Low impurity (residual substance) content


Convenient and Efficient
No transcription required, reducing the experimental cycle
High Editing Efficiency
Increased experimental success rate


No DNA Interference
No unnecessary gene fragment insertion
Stable Quality
Strict quality control between batches


High Synthesis Capacity
Up to 180 nt
Service Details

Service

Modifications

length(nt)

Quantity

Turnaround Time (Business Day)

Purification

QC

Deliverable

Cas9 sgRNA -OPC

PS and 2'-OMe modifications at the first 3 and last 3 nucleotides

97-103

1.5 nmol

4~5

OPC 

UV &MS

· Tube or customized lyophilized RNA
· COA report (electronic)

 

3 nmol

5 nmol

Cas9 sgRNA -HPLC

1.5 nmol

8~10

HPLC

UV & MS & HPLC

3 nmol

5 nmol

Cas9 crRNA

None

-36

5 nmol

3~5

HPLC


UV & MS & HPLC

 

Cas9 tracrRNA

None

-67

5 nmol

3~5

LbaCas12a crRNA

None

-40

5 nmol

3~5

AsCas12a crRNA

None

-40

5 nmol

3~5

Workflow
Free
Design
Free
From nmol to mmol levels
Synthesis
From nmol to mmol levels
OPC or HPLC
Purification
OPC or HPLC
OPC: UV & MS;
HPLC: UV & MS & HPLC
QC
OPC: UV & MS;
HPLC: UV & MS & HPLC
Tube or Customized、Lyophilized RNA、COA Report
Delivery
Tube or Customized、Lyophilized RNA、COA Report
Case

The sample is a 100 nt sgRNA with three 2'-OMe modifications at both ends. Mass spectrometry and chromatography analyses confirm its sequence accuracy and a high purity of 96.54%
         MS Test 
           
          High Purity
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