Name: Custom DNA Oligos
Brand: Tsingke Biotech
SKU: 00304
Availability: InStock
Rating: 5 (1 reviews)

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Home » Product & Services » Oligo Synthesis » Custom DNA Oligos

Overview

DNA Oligos are short, single-stranded DNA molecules, usually consisting of 20 to 30 base pairs. These DNA oligos play a crucial role in various molecular biology applications, including PCR amplification, gene cloning, sequencing, gene expression analysis, and other molecular biology experiments.

Tsingke’s DNA oligos, synthesized using cutting-edge technology, boast high purity levels with 100% sequence accuracy. Each DNA oligo is meticulously monitored during synthesis and controlled according to Tsingke's stringent quality assurance and quality control standards. By integrating synthetic raw materials, equipment, technology, and services, Tsingke ensures a seamless industrial chain, delivering safe, reliable, and cost-effective customized DNA oligos. These custom DNA oligos cater to the needs of scientific research and R&D production across universities, research institutes, hospitals, government agencies, and pharmaceutical diagnostic companies.

Advantages

Various QC Methods

100% mass spectrometry with a variety of additional detection options

Various Modification Types

Over 200 chemical modifications available for your selection

Large-scale

Flexible synthesis specifications, μg~kg delivery ability

Service Details

Service Name	Length (nt)	Purification	Price/ Turnaround time	Deliverable	Application
Common Oligos	5-60	DSL/OPC/PAGE/HPLC	Inquire	· Tube or Customized · Lyophilized DNA · COA Report (electronic)	PCR、DNA Sequencing
Long Oligos	60+	PAGE/HPLC/Dual PAGE & HPLC			NGS, genomic Research
Large-scale Oligos	Customized	Customized			New drug screening, drug production
Modified Oligos	5-120	DSL/OPC/PAGE/HPLC/ Dual PAGE & HPLC			Various molecular biology research

Common modification types

Spacers	Linkers	Fluorophores	Quenchers	Modified Bases	Other Modifications	Degenerate Base
Spacer C3	Acrydite	Alexa 633	BHQ1	2'-F-rA/rC/rG/rU	Cholesteryl	B=G,C,T
Spacer C6	Azide	CY3	BHQ2	2'-O-Methyl A/C/G/U	Ferrocene	D=A,G,T
Spacer C12	Biotin	CY5	BHQ3	inverted dT		H=A,C,T
Spacer 9	Biotin-TEG	CY5.5	Dabcyl	m5C/m5dC		K=G,T
Spacer 12	CHCH	CY7	Eclipse	ddC		M=A,C
Spacer 18	CHO	ET-ROX	MGB	dI		N=A,G,C,T
dSpacer	COOH	ET-TAMRA	XEN	dU		R=A,G
	C6-NH2	FAM		5F-dU		S=G,C
	C7-NH2	HEX		LNA-A/C/G/T		V=A,G,C
	C12-NH2	JOE		m6A/m6dA		W=A,T
	DBCO	PET		Phosphorylation		Y=C,T
	Digoxin	Quasar 570		Phosphorothioate
	Thiol SH	Quasar 670		RNA base
	Thiol SS	ROX
		TAMRA
		TET
		Tsxas Red
		VIC

Workflow

High-throughput synthesizer from pmol to mmol levels

Synthesis

High-throughput synthesizer from pmol to mmol levels

Waters 2695 & Waters 2767 automatic purification and PAGE purification

Purification

Waters 2695 & Waters 2767 automatic purification and PAGE purification

All MASS quality inspection, CE and other additional quality inspections

Automatic dispensing instrument for accurate dispensing

Distribution

Automatic dispensing instrument for accurate dispensing

Tube or Customized、Lyophilized DNA、COA Report

Delivery

Tube or Customized、Lyophilized DNA、COA Report

Case

Figure 1. Coupling efficiency ：99.5%

Figure 2. MS: Molecular weight deviation ≤ 0.05%

Related Resource

Tsingke Oligo(DNA) Synthesis Order Form

Oligo Synthesis Flyer V1.1.1.240129

FAQ

What if mutations were found in the primers when sequencing?

Primer synthesis is a multi-step chemical reaction, each step of the synthesis efficiency is the highest 99%, by-products can not be avoided. The longer the chain, the higher the frequency of mutations adds up. In order to save time and improve the success rate when you clone sequencing after PCR amplification, we have the following suggestions:
(1) Please prepare 2 to 3 bacterial solutions including positive clones after the detection of positive clones, and send 2 or more for sequencing, so that the success rate will be greatly improved, and save a lot of time as well;

(2) One clone can also be sequenced first, and the bacterial solution of the remaining two clones can be stored at 4 degrees in the refrigerator. Once certain point mutations or deletions occur, the remaining two clones can be sequenced immediately;

(3) In this way, the possibility of obtaining the correct sequence is very high, and a series of experimental operations of re-PCR, linking, cloning and screening can be avoided, and a lot of time can be saved.
If you find that more than 2 to 3 clones have mutations in the primer region, and it is confirmed that it is caused by the primer, we will immediately arrange an urgent free coincidence, and send it to your hand as quickly as possible.

Why do long chain primers cost more than short chain primers?

When combining long chain primers, more reagents are required than short chain primers, especially primers longer than 90 base. Due to the increase in cost, the price is higher.

What is the longest primer that can be synthesized?

The longer the primer, the higher the probability of encountering issues. Unless there is a specific requirement, we recommend not exceeding 80 bases in primer length. With the current primer synthesis efficiency, for 80-base products, the percentage of full-length primers does not exceed 40%. Subsequent processing can also result in significant loss, leading to low yields. The maximum length for primer synthesis is more than 120 bases. It’s up to you.

Is there phosphorylation at the 5' end of the synthesized primer?

The 5' end of the synthesized primer is hydroxyl and has no phosphate group. If desired, you can phosphorylate at the 5' end with polynucleotide kinase, or ask us to synthesize directly at 5'. Or the 3' end is phosphorylated at an additional charge.

What are the methods of purification?

The common purification methods are DSL, OPC, PAGE,HPLC and Dual PAGE & HPLC .

What impurities are contained in the crude products of DNA synthesis?

It is mainly the failed fragment produced during the synthesis reaction and the ammonium salt produced when the protective group is removed.

Why is the yield of modified primers lower and the price higher than that of general primers?

The main reason is that the modified monomer has poor stability, long coupling time and low efficiency, and the final yield is naturally lower than that of ordinary primers. Modifying primers usually require PAGE or HPLC purification, purification process loss is large, the raw material used in the modification primer is hundreds of times that of the general primer raw material, so the price of the product is naturally high.

What are the basic principles of primer design?

The length of the primer is generally between 15-30 bases;

The GC content of primers is between 40%-60%.

The bases are randomly distributed;

There should be no complementary sequence between primers and primers.

The △G value at the 5 'end and the middle of the primer should be relatively high, while the △G value at the 3' end should be low.

The single chain of the amplified product could not form the secondary structure.

Primers should be specific.

**For Research Use Only. Not for use in diagnostic procedures.

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