Standard Gene Synthesis

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Standard Gene Synthesis is a cutting-edge service that revolutionizes the process of obtaining target genes compared to conventional molecular cloning methods. Our standard gene synthesis services offer personalized synthesis plans tailored to diverse lengths and application requirements, ensuring customization. By providing us with the DNA sequence you need synthesized, we guarantee prompt delivery of the ideal plasmid containing your target gene. With guaranteed 100% sequence accuracy with Sanger sequencing and NGS, we stand out as the preferred partner for top research institutions worldwide.
The cost-effective solution through Tsingke's comprehensive industrial chain
Guaranteed 100% sequency with Sanger sequencing and NGS
Free 160+ vectors
Free codon optimization

Service Details


*Turnaround time

(Business Day)


<1.5 kb


1 tube of lyophilized plasmid DNA 
(about 1-4 μg/ tube); 

Sequencing map (.abl file);

Target sequence (.seq file);

COA Report(electronic). 

1.5 kb~3.5 kb


3.5 kb~5 kb


5 kb~7 kb


7 kb~10 kb


>10 kb


*Turnaround Time for simple sequences only and may change with the complexity of the gene sequence. Get your accurate estimated turnaround time by emailing
sequence analysis & codon optimization
Sequence Optimization
sequence analysis & codon optimization
design & synthesis
Oligo Synthesis
design & synthesis
overlap PCR & gene assembly
overlap PCR & gene assembly
QC for NGS & Sanger sequencing
Quality Control
QC for NGS & Sanger sequencing
ship the plasmid containing your gene insert
ship the plasmid containing your gene insert
Case 1

Codon Used Adjustment

A CAI of 1.0 is considered to be perfect in the desired expression organism, and a CAI of > 0.75 is regarded as good, in terms of high gene expression level. After optimized CAI : 0.85
Figure 1 The distribution of codon usage relativate frequency
Case 2

GC Content Adjustment

The ideal percentage range of GC content is between 35-65%. In this report, GC content statistics using a 30bp window. After optimized GC: 58.99
Figure 2 The comparison of GC content between original sequence and optimized sequence
Related Resource
Does Tsingke provide codon optimization? Does optimization have an impact on gene expression?
Tsingke offers free codon optimization.
For the same amino acid in different hosts, the corresponding codons may exhibit varying degrees of preference. Codon optimization involves utilizing preferred codons and avoiding rare codons to optimize the expression of a sequence in a specified host. There is a strong correlation between gene expression levels and codon preference. Tsingke's unique GeneOptimizer software utilizes a codon optimization algorithm that supports optimization for tens of thousands of hosts and provides customized services. The main parameters for optimization include codon preference, GC content, restriction enzyme sites (deletion), poly structure, sequence repeats, etc.
Which termination codon is preferred in the E. coli expression system? What about in mammalian systems?
In E. coli, TAG is rarely used; TAA is frequently used; TGA is also available.
In mammals, TGA is used more frequently than TAA and TAG.
Relative to the differences between mammals and E. coli, codon usage tables do not differ very much between different mammalian organisms.
How does Tsingke solve high-difficulty genes, such as those with high or low GC content, highly repetitive sequences, very long sequences, and special
High and low GC content, repetitive sequence:
Solution: Divide the sequence into small segments to reduce the impact of structure on synthesis, and choose the optimal connection solution, such as enzyme digestion and ligation and multi-segment recombination.
Very long sequences:
Solution: use multi-segment recombination to construct divided small segments, and combine them with vitro and vivo assembly.
Special parts of vectors (e.g. ccdB, LTR):
Solution: Use different competent cells corresponding to different parts of vectors, such as DB3.1, which renders the strain resistant to the toxic effects of the ccdB gene, the recombination-deficient Stbl series, etc.
Other unpredictable difficulties, such as genetic instability and toxicity, and the introduction of random mutations and deletions
Solution: Try different competent cells.
Can you keep my sequence information confidential?
Yes, all customer information will be kept confidential.
Which sites in the pUC57 vector will the synthesized gene be cloned?
Generally, the default clone is between EcoRⅠ and Hind Ⅲ. If you have particular needs, please note and we can select other suitable cloning sites according to the experimental needs based on your sequence.
Are there any requirements for my provided-vector?
We need you to ship at least 2 μg plasmid.
What vector does Tsingke use by default? Can I send my vectors to you?What vector does Tsingke use by default? Can I send my vectors to you?
We provide pUC57 vectors containing Amp (ampicillin) for free by default, and we also have a vector library for 160+ options that you could use for free.
Alternatively, you may use your own vector. Simply provide the vector sequence and a vector sample. It is recommended to ship your vector in advance, otherwise, the Turnaround Time (TAT)  may need to be extended by 3 business days to verify the vector.
What information do you need to prepare a quote?
We require information on the insert DNA sequence or amino acid sequence, and the host species if codon optimization is needed. Please ensure that the restriction sites are specified at the 5'/3' end or indicate if any internal modifications need to be avoided. Additionally, let us know if you wish to include other elements in the sequence, such as the Kozak sequence, stop codon, etc. 
Feel free to complete the evaluation form and send it via email to For any inquiries or project requirements, you can contact our account managers or sales managers through email. Our technical support team will promptly assess the price and turnaround time (TAT) and provide you with a detailed quotation as soon as possible."
*For Research Use Only. Not for use in diagnostic procedures.
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