Standard Gene Synthesis

We require the DNA or amino acid sequence you need synthesized.If codon optimization is required, specify the host species. Additionally, indicate any required restriction sites at the 5' and/or 3' ends, any internal modifications to avoid, and if you need additional sequences added (e.g., Kozak sequence, stop codons, etc.).Or you can download the “Tsingke Gene Synthesis Order Form”from our website and email it to info@tsingke.com.cn. Our technical support team will promotly estimate the pricing and turnaround time for you and send you a quotation shortly.

No, the order defaults to providing 4 μg of plasmid dry powder. This plasmid is not recommended for direct transfection into cells or protein expression. If you need to conduct related experiments, please perform plasmid transformation, then isolate a single colony for cultivation, and use a plasmid extraction kit that meets the required standards to extract high-quality plasmid. Our company also provides plasmid extraction services. If you have higher requirements for the plasmid, you can select our plasmid extraction service simultaneously when placing your order.

The synthesized plasmid can be stored for one vear. Customer-provided vectors that are free of issues will also be stored forone year. During the storage period, you can use the plasmid for site-directed mutagenesis, cloning, or other services.

If there are any quality issues within one month of receipt,we will provide a free replacement.Overseas shipping fees will be charged separately, or the plasmids can be sent with other orders.

High and low GC content, repetitive sequence:

Solution: Divide the sequence into small segments to reduce the impact of structure, and choose the optimal connectionsolution, such as enzyme digestion and ligation and multi-segment recombination.

Long sequences:

Solution: use multi-segment recombination to construct divided gene fragments, and combine them with vitro and vivo assembly.

Special parts of vectors (e.g. ccdB, LTR)

Solution: Use different competent cells corresponding to different parts of vectors, such as DB3.1, which renders the strain resistant to the toxic effects of the ccdB gene, the recombination-deficient Stbl series, etc.

Other unpredictable dlificulties, such as genetic instability and toxicity, and the introduction of random mutations and deletions.

Solution: Try different competent cells.

Tsingke ensures sample uniformity by isolating monoconal clones, Each clone is validated by Sanger sequencing, covering the entiregene and at least 50 bp of the vector backbone upstream and downstream, which ensures the gene is correct and properly placed inthe vector. The plasmid then undergoes NGS to verify the accuracy of the entire plasmid and detect any contamination. Beforeshipment, a strict OC review is conducted.