Name: NGS Oligos
Brand: Tsingke Biotech
SKU: 00301
Availability: InStock
Rating: 5 (1 reviews)

Overview

NGS Oligos are used in Next-Generation Sequencing (NGS) technology. These Oligos have different functions such as library construction, amplification and capture, and are important raw materials for NGS.

Tsingke has rich experience in oligonucleotide synthesis, provides NGS products that support two platforms, including hybridization-based enrichment and amplicon-based enrichment, with efficient sealing effects and excellent capture efficiency.Tsingke brings customers high-quality Oligo products with high purity, high quality, and low cross-contamination rate to ensure the accuracy of downstream experiments.

Advantages

High consistency

Low batch-to-batch difference to ensure high homogeneity of multiple target fragments obtained

Low cross-contamination

Stringent purification standards produce higher quality products with less than 0.05% cross-contamination rate

Professional NGS team

High coupling efficiency with dedicated NGS team

Service Details

Service Name	Length (nt)	Purification	Price/ Turnaround time	Deliverable	Application
Adapter	15-80	HPLC/PAGE	Inquire	· Tube or Customized · Lyophilized DNA · COA Report(electronic)	NGS library construction
Blocker	Customized	HPLC			Blocked sample adapter sequence
Capture probes	80-120	HPLC/PAGE			Target area capture
Multiplex PCR Oligos	15-120	HPLC/PAGE			Sequencing of specific target regions

*Note: In addition to the recommended content, Oligo length and purification methods can also be customized.

Workflow

High-throughput synthesizer from pmol to mmol levels

Synthesis

High-throughput synthesizer from pmol to mmol levels

Waters 2695 & Waters 2767 automatic purification and PAGE purification

Purification

Waters 2695 & Waters 2767 automatic purification and PAGE purification

All MASS quality inspection, CE and other additional quality inspections

Automatic dispensing instrument for accurate dispensing

Distribution

Automatic dispensing instrument for accurate dispensing

Tube or Customized、Lyophilized DNA、COA Report

Delivery

Tube or Customized、Lyophilized DNA、COA Report

Related Resource

Tsingke Oligo(DNA) Synthesis Order Form

Oligo Synthesis Flyer V1.1.1.240129

FAQ

What is NGS adapter?

NGS adapter is short sequence of bases, which consists of three parts:
1.Sequences that enable the library to bind and form clusters on the flow cell (p5 and p7 sequences);
2.Binding sites for sequencing primers to initiate sequencing (Rd1 SP and Rd2 SP);
3. Index or Barcod, serve as sample identifiers,Adapters serve as the bridge between the DNA fragments to be sequenced and the flow cell. Once the target sequences are ligated to the adapters, they can be amplified and sequenced on the flow cell.

What are the main types of capture probes?

Cancer research probes, basic research probes, hematologic probes, pathogenic probes, genetic disease probes, methylation probes.

What are the key factors to consider when designing multiplex PCR primers?

1.Primer Length: Primers should be 18-24 bases long to minimize the likelihood of primer dimer formation.
2.Tm Values: The melting temperatures (Tm) of all primers in the multiplex reaction should be similar to ensure uniform annealing.
3.3'-Nucleotide Complementarity: Avoid complementarity at the 3' ends of primers to prevent the formation of primer dimers.
4.Individual Optimization: Optimize each pair of primers independently before pooling them. Once pooled, mix and optimize them sequentially.

Why annealing temperature and cycle number are improtent in multiplex PCR?

The optimal annealing temperature for each primer pair needs to be determined individually, and the lowest Tm value among the primers should be used for efficient annealing in the multiplex reaction. Additionally, using the minimum number of amplification cycles necessary to achieve sufficient product yield helps ensure the efficiency and specificity of the PCR.

Why is it important to optimize reagent concentration and extension time in multiplex PCR?

In multiplex PCR, multiple templates are amplified simultaneously, which can make enzyme and nucleotide concentrations limiting factors. Additionally, the time required to fully synthesize all products increases. Multiplex PCR requires longer extension times compared to single-target PCR to ensure complete synthesis of all products.

How should fluorescent labeled probes be stored?

Multiplex PCR primers should be 18-24 bases long. Longer primers are more likely to form primer dimers.