NGS Oligos

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Overview
NGS Oligos are used in Next-Generation Sequencing (NGS) technology. These Oligos have different functions such as library construction, amplification and capture, and are important raw materials for NGS.

Tsingke has rich experience in oligonucleotide synthesis, provides NGS products that support two platforms, including hybridization-based enrichment and amplicon-based enrichment, with efficient sealing effects and excellent capture efficiency.Tsingke brings customers high-quality Oligo products with high purity, high quality, and low cross-contamination rate to ensure the accuracy of downstream experiments.
Advantages
High consistency
Low batch-to-batch difference to ensure high homogeneity of multiple target fragments obtained
Low cross-contamination
Stringent purification standards produce higher quality products with less than 0.05% cross-contamination rate
Professional NGS team
High coupling efficiency with dedicated NGS team

Service Details

Service Name

Length (nt)

Purification

Price/
Turnaround time

Deliverable

Application

Adapter

15-80

HPLC/PAGE

Inquire

· Tube or Customized

· Lyophilized DNA

· COA Report(electronic)

NGS library construction

Blocker

Customized

HPLC

Blocked sample adapter sequence

Capture probes

80-120

HPLC/PAGE

Target area capture

Multiplex PCR Oligos

15-120

HPLC/PAGE

Sequencing of specific target regions

*Note: In addition to the recommended content, Oligo length and purification methods can also be customized.                                                                         
Workflow
High-throughput synthesizer from pmol to mmol levels
Synthesis
High-throughput synthesizer from pmol to mmol levels
Waters 2695 & Waters 2767 automatic purification and PAGE purification
Purification
Waters 2695 & Waters 2767 automatic purification and PAGE purification
All MASS quality inspection, CE and other additional quality inspections
QC
All MASS quality inspection, CE and other additional quality inspections
Automatic dispensing instrument for accurate dispensing
Distribution
Automatic dispensing instrument for accurate dispensing
Tube or Customized、Lyophilized DNA、COA Report
Delivery
Tube or Customized、Lyophilized DNA、COA Report
Case
Figure 1. MS: Molecular weight deviation ≤ 0.05%




Figure 2. HPLC: Purity ≥ 95%



Figure 3. CV (HPLC):0.28%




Figure 4. CV (OD260):1.69%
Related Resource
FAQ
What are the common types of NGS oligos?
The adapter oligo is usually double-stranded (or U-shaped) and adopts 5' phosphorylation modification; the blocking oligo is single-stranded; the capture probe is single-stranded and multiple premixed, usually 5' biotin modified; the multiplex PCR oligo is usually Single strand multiple premixed.
**For Research Use Only. Not for use in diagnostic procedures.
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