Long DNA Synthesis

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Overview
While traditional chemical methods limit DNA lengths to 200 nucleotides, the demand for kilobase to megabase-level genes, even entire genomes, necessitates the support of advanced in vitro assembly technologies. Tsingke stands at the forefront of overcoming key challenges in genome synthesis, particularly focusing on advancing long DNA synthesis and assembly technologies. 

With years of expertise and ongoing innovation, we've transcended the boundaries of genetic synthesis. Our breakthroughs include mastering the core technology of yeast assembly for multiple segments and large fragments, enabling precise and efficient one-step assembly of DNA fragments in Saccharomyces cerevisiae. We've also achieved industrial-scale production of 50 kb large DNA fragment, with demonstrated capabilities to deliver fragments as long as 200 kb.

Our Gene Factory boasts state-of-the-art long fragment synthesis and assembly technologies. We are dedicated to providing customized solutions for metabolic pathway synthesis, genome synthesis, and assembly, while also supporting DNA digital storage and fundamental life science research. Through our technological advancements, we aim to tackle pressing issues such as food scarcity, drug development, disease treatment optimization, environmental challenges, and innovative data storage solutions.
Advantages
ProLongGene offers unparalleled advantages in the realm of long DNA synthesis, enabling the creation of long DNA sequences and long double strand DNA synthesis that traditional methods simply cannot achieve. With capabilities extending up to 200 kb, our custom DNA synthesis long services ensure high assembly efficiency through one-step assembly in the Saccharomyces cerevisiae system. This precision is validated by rigorous Sanger sequencing and NGS, making our long DNA fragments not only accurate but also ideal for complex genome synthesis projects. By mastering long gene synthesis, we empower researchers to explore vast genetic landscapes, facilitating advancements in metabolic pathway synthesis and addressing global challenges like food scarcity and disease treatment. Delve into the future of genetics with ProLongGene's cutting-edge technologies for creating long DNA molecules and achieving your ultimate research goals.
Up to 200kb
Achieved construction as long as 200 kb
High Assembly Efficiency
One-step assembly of DNA fragments in Saccharomyces Cerevisiae assembly system
Accuracy
Guaranteed with Sanger sequencing and NGS

Service Details

1

Our ProLongGene service is designed to meet the needs of projects requiring long DNA strand synthesis and large DNA fragments. We offer custom DNA synthesis for sequences over 10 kb, with rapid turnaround times to ensure timely project delivery. Each order includes a lyophilized plasmid DNA sample, sequencing map, target sequence, and a comprehensive Certificate of Analysis (COA) report. Our advanced synthesis capabilities, combined with high-precision assembly, make ProLongGene the ideal solution for long DNA sequence synthesis, genome synthesis, and large-scale genetic engineering applications.
Length

Turnaround time

(Business Day)

Vector Deliverables
>10 kb Evaluation pCC1413

1 tube of lyophilized plasmid DNA 
(about 1-4 μg/ tube); 

Sequencing map (.abl file);

Target sequence (.seq file);

COA Report(electronic). 

 

Workflow
sequence analysis & codon optimization
Sequence Optimization
sequence analysis & codon optimization
design & synthesis
Oligo Synthesis
design & synthesis
In Vitro assembly & In Vivo assembly
Construction
In Vitro assembly & In Vivo assembly
QC for NGS & Sanger sequencing
Quality Control
QC for NGS & Sanger sequencing
ship the plasmid containing your long DNA sequence insert
Delivery
ship the plasmid containing your long DNA sequence insert
Case
Figure 1. Long Fragment and Genome Synthesis Based on Saccharomyces Cerevisiae System

Currently, Tsingke has successfully developed the Spore Bacillus One-Step Assembly technology to replace traditional extracellular enzyme ligation methods. This technology allows for the rapid and efficient acquisition of 5 kb DNA sequences, enabling the simultaneous assembly of up to 10 fragments in a single step. This significantly shortens the synthesis cycle, reducing the time required by at least half compared to traditional methods. Additionally, we have optimized the yeast assembly system, greatly enhancing the success rate and transformation efficiency of multi-segment assembly. This optimization enables us to successfully complete the assembly of 200 kb DNA.
Related Resource
FAQ
What is delivered for long DNA synthesis services?
We provide one tube of lyophilized plasmid (stored at -20°C).
On which vectors are the synthesized large fragments cloned? Can specific vectors be used?
Our company defines long DNA sequences as those exceeding 10 kb. For sequences ranging from 10 kb to 30 kb, yeast assembly methods are used to first construct them into the pcc1413 vector before transferring them to your specified target vector. Sequences exceeding 30 kb will be directly constructed into the pcc1413 vector.
**For Research Use Only. Not for use in diagnostic procedures.
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