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Plasmid Preparation

Plasmid Preparation

Streamlined services for Plasmid Preparation (plasmid only) or Gene-to-Plasmid delivery, supporting research and transfection applications.
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Plasmid Preparation

Plasmid Preparation
Overview

Tsingke provides plasmid preparation services, including Plasmid Preparation(plasmid only) and Gene to Plasmid, with a complete workflow from sequence design to delivery. Our integrated platforms ensure high-quality, high-purity, and reliable plasmids for diverse research needs.

Key Advantages of Plasmid Preparation

High Quality & Purity
High Quality & Purity

Plasmids suitable for in vitro and in vivo experiments.

Flexible Options
Flexible Options

Choice of backbone and cloning strategies to suit project needs.

Fast Turnaround
Fast Turnaround

Rapid delivery of plasmids for timely research.

Reliable Verification
Reliable Verification

Sequencing-based QC ensures correct sequence and integrity.

Service Details of Plasmid Preparation


TypeIntroduction
Plasmid Preparation

Deliver 100 μg~100 mg Prepared lyophilized plasmid DNA

Corresponding quantities of lyophilized plasmid DNA and COA Report

Research Grade or Transfection Grade (endotoxin-free<0.1 Eu/ μg, 0.01 Eu/ μg,<0.005 Eu/ μg)

Gene to Plasmid

Full-process gene synthesis to plasmid delivery


Workflow of Plasmid Preparation

Culture Growth

Inoculation and expansion of bacterial culture from stock or colony.

Culture Growth

Inoculation and expansion of bacterial culture from stock or colony.

Harvest & Lysis

Bacterial pellet collection and cell lysis to release plasmid DNA.

Harvest & Lysis

Bacterial pellet collection and cell lysis to release plasmid DNA.

Purification

High-quality plasmid purification using column-based technology; endotoxin removal is available.

Purification

High-quality plasmid purification using column-based technology; endotoxin removal is available.

Quality Control

Concentration, purity analysis, and integrity confirmation via gel electrophoresis.

Quality Control

Concentration, purity analysis, and integrity confirmation via gel electrophoresis.

Delivery

Shipped as purified plasmid DNA with a quality control report.

Delivery

Shipped as purified plasmid DNA with a quality control report.

Application of Plasmid Preparation

Cloning
Cloning

Provides high-purity plasmid DNA for efficient vector construction and genetic modification.

Cell Transfection
Cell Transfection

Delivers endotoxin-free, transfection-ready plasmids for high-efficiency gene expression studies.

FAQs of Plasmid Preparation

What is Tsingke’s Plasmid Preparation Service?

Tsingke provides high-quality plasmid extraction services with  Research Grade and Transfection Grade to meet diverse experimental needs.

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