Name: Plasmid Preparation
Brand: Tsingke Biotech
SKU: 00283
Availability: InStock
Rating: 5 (1 reviews)

Advantages

One-stop Solutions

Tsingke provides a comprehensive service encompassing gene synthesis, vector construction, and plasmid preparation

Flexible Customized

Microgram to gram scale quantity

High Industrial Standard

Maximal transfection efficiency with high supercoil content and low endotoxin level

Service Details

Research Grade
Volume	*Turnaround Time(Business Day)	Deliverables
100 μg	5	Prepared lyophilized plasmid DNA; Sequencing map (.abl file); COA Report(electronic).
200 μg	5
500 μg	7
1 mg	7
2 mg	8
3 mg	9
4 mg	10
5 mg	11
10 mg	13
20 mg	13
50 mg	15
100 mg	18

*Opt for Gene Synthesis Alongside Plasmid Preparation and Save 2 Business Days on Turnaround Time

Get your quote by emailing gene@tsingke.com.cn

Quality Control
Research Grade	Items	Method	Specifications
	Appearance	Visualinspection	Colorless Liquid of Pure Transparency
	A260/280 ratio	UV Absorbance	1.8~2.0
	Supercoil content	Agarose gel electrophoresis	＞50%
	Concentration	UV Absorbance	90%~110%
	Residual DNA	Agarose gel electrophoresis	Not visible
	Residual RNA	Agarose gel electrophoresis	Not visible
	Restriction enzyme analysis	Agarose gel electrophoresis	Adjustable according to requirement
	Sequence verification	Sanger sequencing	Consistent with the confirmed plasmid sequence

Transfection Grade
Endotoxin	Volume	*Turnaround Time(Business Day)	Deliverables
＜0.1 Eu/ μg	100 μg	5	Prepared lyophilized plasmid DNA; Sequencing map (.abl file); COA Report(electronic).
	200 μg	5
	500 μg	7
＜0.01 Eu/ μg	1 mg	7
	2 mg	8
	3 mg	9
	4 mg	10
	5 mg	11
＜0.005 Eu/ μg	10 mg	13
	20 mg	15
	50 mg	17
	100 mg	19

*Opt for Gene Synthesis Alongside Plasmid Preparation and Save 2 Business Days on Turnaround Time

Get your quote by emailing gene@tsingke.com.cn

Quality Control
Transfection Grade	Items	Method	Specifications
	Appearance	Visual inspection	Colorless Liquid of Pure Transparency
	A260/280 ratio	UV Absorbance	1.8~2.0
	Supercoil content	Agarose gel electrophoresis	＞85%
	Concentration	UV Absorbance	90%~110%
	Residual DNA	Agarose gel electrophoresis	Not visible
	Residual RNA	Agarose gel electrophoresis	Not visible
	Restriction enzyme analysis	Agarose gel electrophoresis	Adjustable according to requirement
	Sequence verification	Sanger sequencing	Consistent with the confirmed plasmid sequence
	Endotoxin	Limulus amebocyte lysate (LAL) test	Endotoxin ≤ Standard
	Exogenous Contamination Detection	NGS(Depth>30×)	Genomic DNA < 1% Other DNA Contamination < 0.1%

FAQ

Does Tsingke provide codon optimization? Does optimization have an impact on gene expression?

Tsingke offers free codon optimization.

For the same amino acid in different hosts, the corresponding codons may exhibit varying degrees of preference. Codon optimization involves utilizing preferred codons and avoiding rare codons to optimize the expression of a sequence in a specified host. There is a strong correlation between gene expression levels and codon preference. Tsingke's unique GeneOptimizer software utilizes a codon optimization algorithm that supports optimization for tens of thousands of hosts and provides customized services. The main parameters for optimization include codon preference, GC content, restriction enzyme sites (deletion), poly structure, sequence repeats, etc.

Which termination codon is preferred in the E. coli expression system? What about in mammalian systems?

In E. coli, TAG is rarely used; TAA is frequently used; TGA is also available.

In mammals, TGA is used more frequently than TAA and TAG.

Relative to the differences between mammals and E. coli, codon usage tables do not differ very much between different mammalian organisms.

How does Tsingke solve high-difficulty genes, such as those with high or low GC content, highly repetitive sequences, very long sequences, and special

High and low GC content, repetitive sequence:

Solution: Divide the sequence into small segments to reduce the impact of structure on synthesis, and choose the optimal connection solution, such as enzyme digestion and ligation and multi-segment recombination.
Very long sequences:

Solution: use multi-segment recombination to construct divided small segments, and combine them with vitro and vivo assembly.

Special parts of vectors (e.g. ccdB, LTR):

Solution: Use different competent cells corresponding to different parts of vectors, such as DB3.1, which renders the strain resistant to the toxic effects of the ccdB gene, the recombination-deficient Stbl series, etc.

Other unpredictable difficulties, such as genetic instability and toxicity, and the introduction of random mutations and deletions

Solution: Try different competent cells.

Can you keep my sequence information confidential?

Yes, all customer information will be kept confidential.

Which sites in the pUC57 vector will the synthesized gene be cloned?

Generally, the default clone is between EcoRⅠ and Hind Ⅲ. If you have particular needs, please note and we can select other suitable cloning sites according to the experimental needs based on your sequence.

Are there any requirements for my provided-vector?

We need you to ship at least 2 μg plasmid.

What vector does Tsingke use by default? Can I send my vectors to you?What vector does Tsingke use by default? Can I send my vectors to you?

We provide pUC57 vectors containing Amp (ampicillin) for free by default, and we also have a vector library for 160+ options that you could use for free.
Alternatively, you may use your own vector. Simply provide the vector sequence and a vector sample. It is recommended to ship your vector in advance, otherwise, the Turnaround Time (TAT) may need to be extended by 3 business days to verify the vector.

What information do you need to prepare a quote?

We require information on the insert DNA sequence or amino acid sequence, and the host species if codon optimization is needed. Please ensure that the restriction sites are specified at the 5'/3' end or indicate if any internal modifications need to be avoided. Additionally, let us know if you wish to include other elements in the sequence, such as the Kozak sequence, stop codon, etc.
Feel free to complete the evaluation form and send it via email to info@tsingke.com.cn. For any inquiries or project requirements, you can contact our account managers or sales managers through email. Our technical support team will promptly assess the price and turnaround time (TAT) and provide you with a detailed quotation as soon as possible."