ASO

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Overview

Antisense oligonucleotides (ASOs) are short, synthetic, single-stranded oligodeoxynucleotides that typically contain 15 to 25 monomers. They are designed to specifically target mRNA and inhibit its translation into proteins.

Upon cellular entry, ASOs can trigger mRNA degradation by specifically binding to complementary target sequences, facilitated by ribonuclease H1 (RNase H1) activity, consequently suppressing protein expression. Alternatively, ASOs can modulate gene translation by exerting steric hindrance effects on pre-mRNA, thereby achieving selective splicing and contributing to therapeutic interventions for various diseases.

Tsingke provides comprehensive ASO synthesis services with strict adherence to ISO 13485:2016 quality standards to ensure the delivery of high-quality products. We offer a wide range of modifications to enhance oligo stability, as well as flexibility in synthesis scale from micrograms to kilograms. Our purification options, including ESI mass spectrometry and HPLC, are designed to meet your specific experimental requirements.

Advantages
Comprehensive modifications
LNA、cEt、2’-O-C16、、2’-MOE、2’-Ome、psiU、N1-psiU、PS、FAM、Fluorophores modifications


High Quality
Customizable QC standards, HPLC purity up to 99%
Flexible
Synthesis scale from micrograms to kilograms, flexible packaging options, and precise quantification



Customizable Testing
A wide range of testing options available to meet requirements from research to GMP-grade production
High Standard
ISO 13485:2016,  from RUO to GMP compliance




Cost-effective solution
Leverage Tsingke's whole industry chain from consumables, oligo synthesizer  to synthesis  platform
Service Details
Product

Modification

Purification

QC

Deliverable

Custom ASO Synthesis

LNA, cEt, 2’-O-C16,
2’-MOE, 2’-Ome, psiU,
N1-psiU, PS, FAM,
Fluorophores modifications

RNase free HPLC

MS

optional: HPLC,

SEC-HPLC

· Tube or customized lyophilized RNA

· COA report

 (electronic)

Commercialization
During the drug production phase, we rigorously adhere to GMP requirements to guarantee the high standards of quality and safety. We provide technical support encompasses  the production process, ensuring robust compliance with regulatory and standard protocols, make the entire drug development pipeline  compliant ,  safe and efficacious.

Stage

Support Provided

Preclinical Research

- Provide products for pharmacological and toxicological studies

- Assist in completing pharmacological and toxicological research

- Ensure the safety and efficacy of candidate drugs in animal models

Phase Ⅰ

-Assist in completing pharmaceutical trials and initial studies

- Write submission materials

- Provide IND application services

Phase Ⅱ

- Help determine the efficacy and dose-response relationships of new drugs

Phase Ⅲ

- Assist in completing pharmaceutical research

- Prepare for new drug market entry

- Offer documentation writing and submission services

Commercialization

- Provide support for commercial production

- Ensure stable and efficient market supply

- Adhere to national and international regulations and standards throughout the drug development process

Customizable Testing
RUO: MW, SEC-HPLC
GMP: Customizable QC standards, OLIGO content, sequence, water content, residual solvents, elemental impuruties, endotoxin, bioburden
Workflow
High-throughput synthesizer from pmol to mmol levels Purification: RNase free HPLC
Synthesis
High-throughput synthesizer from pmol to mmol levels Purification: RNase free HPLC
MS
QC
MS
(optional)
HPLC/SEC-HPLC
(optional)
Automatic dispensing instrument for accurate dispensing
Distribution
Automatic dispensing instrument for accurate dispensing
Tube or Customized、Lyophilized RNA、COA Report
Delivery
Tube or Customized、Lyophilized RNA、COA Report
Case
2’-MOE and PS Modifications
LNA and PS Modifications
FAQ
What do I need to provide for PCR cloning services?
a.Templates:  Provide and send us the template plasmid. If the plasmid was constructed by Tsingke and the order was placed within the last year, you do not need to provide it again.
b.Vector details:  Choose from our 160+ free vectors or provide your vector.
c.Provide the sequences and resistance information for both template and vector, along with the target sequence and cloning site information.
d.Order Form: Download the "Tsingke Gene Synthesis Order Form" from our website, fill in the relevant fields according to your needs, and send it via email to gene@tsingke.com.cn to place your order. the 
a.Templates:  Provide and send us the template plasmid. If the plasmid was constructed by Tsingke and the order was placed within the last year, you do not need to provide it again.b.Vector details:  Choose from our 160+ free vectors or provide your vector.c.Provide the sequences and resistance information for both template and vector, along with the target sequence and cloning site information.d.Order Form: Download the "Tsingke Gene Synthesis Order Form" from our website, fill in the relevant fields according to your needs, and send it via email to gene@tsingke.com.cn to place your order.
What is the recommended transfection concentration for ASO?
The recommended initial transfection concentration for ASO is 50 nM, with detection times of 24-72 hours. Optimal concentration typically falls between 10-100 nM and should be determined through gradient screening.
How can I improve the silencing efficiency of ASO?
Increase transfection efficiency: Optimize transfection conditions, cell type, and transfection reagents.
Optimize ASO concentration: Design a wide range of ASO concentration gradients to test and find the most suitable one.
Change the ASO sequence: Consider alternative sequences if secondary structures at the target site limit effectiveness.
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