Oligo Synthesis

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Overview
Oligos (or oligonucleotides) , short chains of nucleotides ranging from a few bases to several dozen, are versatile tools in molecular biology. Their length can be tailored to research requirements, with scalable production to meet specific quantity needs. Oligos are commonly used as primers in PCR (Polymerase Chain Reactions) to selectively amplify target DNA sequences. Furthermore, they can be customized with modifications for applications in gene sequencing and diagnosis studies.

Tsingke’s Oligo Synthesis services offer a versatile options to cater to the evolving needs of molecular bench scientists. With over 200 chemical modifications available and multiple purification options, our oligo synthesis services ensure the high-quality results you demand. Tsingke’s Oligo Synthesis stands out with ISO 13485 certification from SGS, serving over 500 IVD companies. We employ various QC methods, including 100% mass spectrometry analysis and additional detection options, ensuring top-notch accuracy and reliability in every oligo product.
Advantages
ISO 13485
ISO 13485
100,000-level clean room and ISO 13485 certification provided by SGS
Customer Trust
Customer Trust
Serving over 500 IVD companies
Various QC Methods
Various QC Methods
100% mass spectrometry with a variety of additional detection options
Service Type

With a comprehensive industrial chain in nucleic acids synthesis, we provide various oligo products, including Custom DNA Oligos, Custom RNA Oligos, qPCR probes, and NGS oligos, suitable for both basic research. Trust Tsingke to provide high-quality oligo synthesis products tailored to meet your specific needs with precision and reliability!

Service Details
Custom DNA Oligos
- Over 200 chemical modifications for your selection.
- Flexible synthesis specifications,μg~kg delivery ability.
- 100% mass spectrometry with a variety of additional detection options.
Custom RNA Oligos
- Single-stranded, duplexed RNA or RNA/DNA chimeras synthesized to your specifications.
- Mature technology and raw materials, strong RNA stability.
- Flexible synthesis specifications,μg~kg delivery ability.
qPCR Probes
- Low exogenous contamination,avoid NTC interference for credible results.
- Stable fluorescent modification raw materials and processes.
- Multiple enhanced purification methods and customized QC.
NGS Oligos
- Adapter cross-contamination rate ≤0.05%.
- Low batch-to-batch difference to ensure high homogeneity of the same product.
- Large-scale automatic purification with minute equipment to ensure accur.
Facility
Facility
Facility
Facility
Facility
Facility
Related Resource
FAQ
What information do I need to provide to assess sequence pricing and turnaround time?

We require the DNA or amino acid sequence you need synthesized. If codon optimization is required, specify the host species. Additionally, indicate any required restriction sites at the 5' and/or 3' ends, any internal modifications to avoid, and if you need additional sequences added (e.g., Kozak sequence, stop codons, etc.).Or you can download the "Tsingke Gene Synthesis Order Form" from our website and email it to info@tsingke.com.cn. Our technical support team will promptly estimate the pricing and turnaround time for you and send you a  quotation shortly.

What contaminants are present in the Crude Product?

Crude oligonucleotides contain the desired product, failure sequences and chemically modified by-products.

Do Tsingke’s oligos come with 5' phosphorylation

Synthesized oligos have a hydroxyl group at the 5' end and are not phosphorylated. 

If 5' phosphorylation is required, you can phosphorylate them using polynucleotide kinase, or request phosphorylation at the 5' or 3' end when palce your order.

What is the maximum length Tsingke can synthesize oligos?

We specialize in synthesizing RNA oligos up to 180 nucleotides and DNA oligos exceeding 250 nucleotides.

How to Store Oligos?

Oligo primers in freeze-dried state are highly stable and can be stored at -20°C for at least one year. After reconstitution, dissolved oligos can be stored as a 100 μmol/L stock solution, aliquoted into multiple tubes, and kept at -20°C for at least six months (frequent freeze-thaw cycles may reduce shelf life). Before use, dilute the concentrated solution to the working concentration for your experiments.

How to Dissolve Oligos?
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Each OD of primer needs to be diluted with water to a concentration of 100 μmol/L (equivalent to 100 pmol/μL).You could add an appropriate amount of nuclease-free double-distilled water (pH >6.0) or TE buffer (pH 7.5-8.0) according to your experimental needs.

Before opening the vial, centrifuge it at 3000-4000 rpm for 1 minute to prevent the loss of primers when opening.

What is the Principle of Oligo Synthesis and What Types of Services are Offered?

Oligo synthesis utilizes solid-phase phosphoramidite chemistry. The main types of oligonucleotides we synthesize include:

DNA oligos

RNA oligos

Probes

siRNA

sgRNA

miRNA

ASOs 

Various modified oligos

*For Research Use Only. Not for use in diagnostic procedures.
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