Crude oligonucleotides contain the desired product, failure sequences and chemically modified by-products.
With a comprehensive industrial chain in nucleic acids synthesis, we provide various oligo products, including Custom DNA Oligos, Custom RNA Oligos, qPCR probes, and NGS oligos, suitable for both basic research. Trust Tsingke to provide high-quality oligo synthesis products tailored to meet your specific needs with precision and reliability!
| Custom DNA Oligos |
- Over 200 chemical modifications for your selection.
- Flexible synthesis specifications,μg~kg delivery ability. - 100% mass spectrometry with a variety of additional detection options.
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| Custom RNA Oligos |
- Single-stranded, duplexed RNA or RNA/DNA chimeras synthesized to your specifications.
- Mature technology and raw materials, strong RNA stability. - Flexible synthesis specifications,μg~kg delivery ability. |
| qPCR Probes |
- Low exogenous contamination,avoid NTC interference for credible results.
- Stable fluorescent modification raw materials and processes. - Multiple enhanced purification methods and customized QC.
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| NGS Oligos |
- Adapter cross-contamination rate ≤0.05%.
- Low batch-to-batch difference to ensure high homogeneity of the same product.
- Large-scale automatic purification with minute equipment to ensure accur.
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We require the DNA or amino acid sequence you need synthesized. If codon optimization is required, specify the host species. Additionally, indicate any required restriction sites at the 5' and/or 3' ends, any internal modifications to avoid, and if you need additional sequences added (e.g., Kozak sequence, stop codons, etc.).Or you can download the "Tsingke Gene Synthesis Order Form" from our website and email it to info@tsingke.com.cn. Our technical support team will promptly estimate the pricing and turnaround time for you and send you a quotation shortly.
Crude oligonucleotides contain the desired product, failure sequences and chemically modified by-products.
Synthesized oligos have a hydroxyl group at the 5' end and are not phosphorylated.
If 5' phosphorylation is required, you can phosphorylate them using polynucleotide kinase, or request phosphorylation at the 5' or 3' end when palce your order.
We specialize in synthesizing RNA oligos up to 180 nucleotides and DNA oligos exceeding 250 nucleotides.
Oligo primers in freeze-dried state are highly stable and can be stored at -20°C for at least one year. After reconstitution, dissolved oligos can be stored as a 100 μmol/L stock solution, aliquoted into multiple tubes, and kept at -20°C for at least six months (frequent freeze-thaw cycles may reduce shelf life). Before use, dilute the concentrated solution to the working concentration for your experiments.
Each OD of primer needs to be diluted with water to a concentration of 100 μmol/L (equivalent to 100 pmol/μL).You could add an appropriate amount of nuclease-free double-distilled water (pH >6.0) or TE buffer (pH 7.5-8.0) according to your experimental needs.
Before opening the vial, centrifuge it at 3000-4000 rpm for 1 minute to prevent the loss of primers when opening.
Oligo synthesis utilizes solid-phase phosphoramidite chemistry. The main types of oligonucleotides we synthesize include:
DNA oligos
RNA oligos
Probes
siRNA
sgRNA
miRNA
ASOs
Various modified oligos
