RNA interference (RNAi) is a phenomenon that inhibits the expression of specific genes in organisms. When double-stranded RNA (dsRNA) homologous to the endogenous mRNA coding region is introduced into the cell, it triggers the degradation of the target mRNA, leading to gene silencing.
After the exogenous dsRNA enters the cell, it generates small interfering RNAs (siRNAs). Small interfering RNAs (siRNAs) are double-stranded RNA molecules typically 21-23 nucleotides in length (19-25 nucleotides is acceptable according to the literature). These siRNAs usually have two nucleotide overhangs at the 3' ends. The antisense strand of these siRNAs, along with various nucleases, forms the RNA-induced silencing complex (RISC), which has the ability to bind to and cleave mRNA, thereby mediating the RNA interference process.
Tsingke has a complete oligonucleotide synthesis platform and can provide customers with conventional siRNA and various modified siRNA synthesis services, ranging from microgram to kilogram quantities. The company strictly adheres to ISO 13485:2016 quality control standards and ensures the delivery of high-quality siRNA to customers through multiple quality control methods, such as ESI mass spectrometry and HPLC purity testing.
Service |
Details |
length(nt) |
Quantity |
Turnaround Time (Business Day) |
Purification |
QC |
Deliverable |
siRNA |
Custom synthesis |
20-25 |
5 nmol or 2 OD |
3-4 |
RNase free HPLC |
HPLC |
· Tube or customized · COA report (electronic) |
siRNA NC |
Various types available: negative/positive control siRNA, transfection control siRNA, etc. |
In-stock |
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3 siRNA, 1 Guaranteed |
With a transfection efficiency of 80%, at least one out of the three siRNAs should achieve an mRNA interference efficiency of over 70%. |
5 nmol or 2 OD |
3-4 |
HPLC | |||
4 siRNA, 1 Guaranteed |
With a transfection efficiency of 80%, at least one out of the four siRNAs should achieve an mRNA interference efficiency of over 70%. |
5 nmol or 2 OD |
3-4 |
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Large-scale siRNA |
Up to kg level |
≥20 nmol or |
Evaluation |