Time-consuming and inefficient traditional cloning methods
Difficulty in constructing plasmids with multiple gRNA expression cassettes
Vector-associated toxicity and off-target expression
Custom plasmids with U6 or other promoters for gRNA transcription.
Vectors for constitutive or inducible Cas9 expression.
Plasmids designed to express multiple gRNAs from a single construct.
Vectors with fluorescent reporters or antibiotic resistance for enrichment.
Automated platforms for fast and accurate plasmid construction.
Full-length sequencing to guarantee plasmid integrity.
Wide range of standard and custom backbones available.
Assistance from design to delivery for your specific application.
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