Low HDR efficiency compared to NHEJ
Difficulty in delivering large DNA templates into cells
Toxicity associated with double-stranded DNA donors
Double-stranded DNA fragments with homology arms for larger insertions.
Plasmid donors for complex or large-scale modifications.
HPLC-purified donors free of contaminants that inhibit HDR.
Expertly designed homology arms to enhance recombination rates.
Available as oligonucleotides, fragments, or cloned vectors.
Comprehensive QC including sequencing to ensure sequence accuracy.
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