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AAV Packaging

AAV Packaging

High-purity single- and double-stranded AAV vectors with tailored titers, supporting overexpression, knockdown (shRNA), and gene knockout (CRISPR-SaCas9) for reliable experimental results.
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Highlights of AAV Packaging

Highlights of AAV Packaging

Adeno-associated virus (AAV) is a uniquely versatile vector, valued for its ability to efficiently transduce a wide range of mammalian cells while being naturally non-pathogenic in humans and exhibiting low immunogenicity. Our custom AAV packaging services deliver high-purity single-and double-stranded vectors optimized for overexpression, shRNA-mediated knockdown, and CRISPR-SaCas9 gene editing. Leveraging proprietary technologies and reagents, we ensure superior viral titers, purity, potency, and consistency for reliable and reproducible experimental outcomes. Flexible packaging options accommodate both standard and ultra-low yield serotypes. Trusted by researchers worldwide, our AAV vectors accelerate gene function studies, streamline workflows, and support advanced functional genomics projects.

Service Details of AAV Packaging


Adeno-Associated Virus (AAV) PackagingTiterTurnaround TimeDeliverables
Ultra-purified ssAAV packaging (Regular-yield serotype)5E+12 VGS12~18  business days (turnaround time of plasmid construction not included)Shipment Items
(1) Custom AAV: Delivered per customer's requested quantity and aliquots; shipping list shows actual titer.

(2) Control AAV: Delivered per customer's requested quantity and aliquots; shipping list shows actual titer.


Electronic reports

(1) COA
(2) Instructions for use
(3) Shipping list
1E+13  VGS
2E+13 VGS
5E+13 VGS
1E+14 VGS
Ultra-purified ssAAV packaging (Ultra-low-yield serotype)1E+12VGS
2E+12 VGS
5E+12 VGS
1E+13 VGS
2E+13 VGS
5E+13 VGS
1E+14 VGS
AAV RNAi (shRNA) / Knockout (CRISPR-SaCas9), Three targets (Regular yield)5E+11VGS
1E+12VGS
5E+12VGS
1E+13VGS
2E+13VGS
5E+13VGS
AAV RNAi (shRNA) / Knockout (CRISPR-SaCas9), Three targets (Ultra-low yield)5E+11VGS
1E+12VGS
5E+12VGS
1E+13VGS
2E+13VGS
5E+13VGS

Ultra-purified scAAV packaging
5E+11 VGS
1E+12 VGS
2E+12 VGS
5E+12 VGS
1E+13 VGS
2E+13 VGS
5E+13 VGS
1E+14 VGS
2E+14 VGS


Cases of AAV Packaging

In Vitro Applications: Tsingke AAV viruses of different serotypes show good infection efficiency across various cell types.

Cases
Cases

FAQs of AAV Packaging

The target cells can be transduced by AAV, but why is the GFP fluorescence intensity very low?

The GFP fluorescence intensity in target cells depends on several factors, including the number of viral particles transducing the cells, the proliferative state of the cells, the cell type, and the observation time. Generally, the higher the number of viral particles transducing the target cells, and the faster the cell proliferation, the stronger the GFP fluorescence. For cells with faster proliferation, GFP expression typically peaks 48 to 72 hours after viral transduction. For slower proliferating cells, the GFP expression time will be extended.

The transduction efficiency of AAV in target cells is low. How can the virus transduction efficiency be improved?
What is the packaging capacity of AAV viruses?
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