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Custom shRNA

Custom shRNA

Design and synthesis of highly effective shRNA constructs for targeted gene silencing in your research.
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Highlights of Custom shRNA

Highlights of Custom shRNA

Leveraging advanced gene synthesis technology, we provide precision-engineered RNAi vectors for reliable gene silencing. Our service includes professional shRNA target design and validated efficiency-guaranteed packages. Clients can choose from multiple preparation formats, including endotoxin-free, ready-to-use liquid plasmids suitable for direct transfection, accelerating your functional genomics research.

Service Details of Custom shRNA


Product nameTurnaround time (Calendar Day)FeaturesDeliverables
Standard - Custom sequence (Design One Target)5~8No guarantee of efficacy; includes free NC (negative control) plasmid.

Lyophilized plasmid: 1 tube (4 µg, NOT endotoxin-free); Stab culture: 1 tube; NC plasmid: 1 tube (15 µg, 1 μg/μL, endotoxin-free).

Standard - Custom sequence (Sequence provided by Customer)5~8No guarantee of efficacy; includes free NC plasmid.

Lyophilized plasmid: 1 tube (4 µg, NOT endotoxin-free); Stab culture: 1 tube; NC plasmid: 1 tube (15 µg, 1 μg/μL, endotoxin-free).

Standard - Package B (3 gene specific shRNAs)5~8With 90% transfection efficiency, at least one shRNA plasmid will achieve 70% mRNA interference. Includes free NC plasmid.

Lyophilized plasmid: 3 tubes (4 µg/tube, NOT endotoxin-free); Stab culture: 3 tubes; NC plasmid: 1 tube (15 µg, 1 μg/μL, endotoxin-free).

Standard - Package A (4 gene specific shRNAs)5~8With 90% transfection efficiency, at least one shRNA plasmid will achieve 70% mRNA interference. Includes free NC plasmid.

Lyophilized plasmid: 4 tubes (4 µg/tube, NOT endotoxin-free); Stab culture: 4 tubes; NC plasmid: 1 tube (15 µg, 1 μg/μL, endotoxin-free).

Ready-to-use -Custom sequence (Design One Target)8~11No guarantee of efficacy; includes free NC plasmid.

Liquid plasmid: 1 tube (100 µg, 1 μg/μL, endotoxin-free); Stab culture: 1 tube; NC plasmid: 1 tube (100 µg, 1 μg/μL, endotoxin-free).

Ready-to-use - Custom sequence (Sequence provided by Customer)8~11No guarantee of efficacy; includes free NC plasmid.

Liquid plasmid: 1 tube (100 µg, 1 μg/μL, endotoxin-free); Stab culture: 1 tube; NC plasmid: 1 tube (100 µg, 1 μg/μL, endotoxin-free).

Ready-to-use - Package B (3 gene specific shRNAs)8~11With 90% transfection efficiency, at least one shRNA plasmid will achieve 70% mRNA interference. Includes free NC plasmid.

Liquid plasmid: 3 tubes (100 µg/tube, 1 μg/μL, endotoxin-free); Stab culture: 3 tubes; NC plasmid: 1 tube (100 µg, 1 μg/μL, endotoxin-free).

Ready-to-use - Package A (4 gene specific shRNAs)8~11With 90% transfection efficiency, at least one shRNA plasmid will achieve 70% mRNA interference. Includes free NC plasmid.

Liquid plasmid: 4 tubes (100 µg/tube, 1 μg/μL, endotoxin-free); Stab culture: 4 tubes; NC plasmid: 1 tube (100 µg, 1 μg/μL, endotoxin-free).

shRNA (NC)In StockThe control contains an NC sequence and is not an empty vector.

Liquid shRNA control plasmid: 1 tube (15 µg); Stab culture: 1 tube.


Ordering Steps of Custom shRNA

Download the order form right and email it to info@tsingke.com.cn,or "Send Your Request" to submit your inquiry online.

Send Your Request
  • Tsingke Custom shRNA Vector Order Form

FAQs of Custom shRNA

Why choose Tsingke’s Custom shRNA Service?

Efficient construction: Rapid generation of shRNA expression plasmids via gene synthesis, eliminating tedious cloning steps.

Professional optimization: Customized design for any gene from any species, with sequence optimization to enhance knockdown efficiency and minimize off-target effects.

Versatile vectors: A wide range of lentiviral backbones available; shRNA can be delivered by direct transfection or packaged into lentiviral particles for infection.

Easy selection: Vectors carry common resistance markers (e.g., PURO/NEO) for stable cell line establishment; fluorescent markers (e.g.,GFP/mcherry) allow real-time monitoring of transfection/infection efficiency and enable flow cytometry sorting.

What information do I need to provide for custom shRNA design?
Does Tsingke provide viral packaging services for shRNA?
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