Leveraging advanced gene synthesis technology, we provide precision-engineered RNAi vectors for reliable gene silencing. Our service includes professional shRNA target design and validated efficiency-guaranteed packages. Clients can choose from multiple preparation formats, including endotoxin-free, ready-to-use liquid plasmids suitable for direct transfection, accelerating your functional genomics research.
| Product name | Turnaround time (Calendar Day) | Features | Deliverables |
| Standard - Custom sequence (Design One Target) | 5~8 | No Effectiveness Guaranteed, NC Plasmid | Lyophilized plasmid: 1 tube (4 µg); Stab culture: 1 tube; Negative control plasmid: 1 tube (15 µg). |
| Standard - Custom sequence (Sequence provided by Customer) | 5~8 | No Effectiveness Guaranteed, NC Plasmid | Lyophilized plasmid: 1 tube (4 µg); Stab culture: 1 tube; Negative control plasmid: 1 tube (15 µg). |
| Standard - Package B (3 gene specific shRNAs) | 5~8 | With 90% transfection efficiency, at least one shRNA plasmid will achieve 70% mRNA interference. | Lyophilized plasmid: 3 tubes (4 µg/tube); Stab culture: 3 tubes; Negative control plasmid: 1 tube (15 µg). |
| Standard - Package A (4 gene specific shRNAs) | 5~8 | With 90% transfection efficiency, at least one shRNA plasmid will achieve 70% mRNA interference. | Lyophilized plasmid: 4 tubes (4 µg/tube); Stab culture: 4 tubes; Negative control plasmid: 1 tube (15 µg). |
| Ready-to-use -Custom sequence (Design One Target) | 8~11 | 1. No Effectiveness Guaranteed, NC Plasmid. 2. Shipping: 100 µg endotoxin-free plasmid (liquid), 15 µg endotoxin-free negative control plasmid (dry powder), ready for direct use in cell transfections. | Liquid plasmid: 1 tube (100 µg); Stab culture: 1 tube; Negative control plasmid: 1 tube (15 µg). |
| Ready-to-use - Custom sequence (Sequence provided by Customer) | 8~11 | 1. No Effectiveness Guaranteed, NC Plasmid. 2. Shipping: 100 µg endotoxin-free plasmid (liquid), 15 µg endotoxin-free negative control plasmid (dry powder), ready for direct use in cell transfections. | Liquid plasmid: 1 tube (100 µg); Stab culture: 1 tube; Negative control plasmid: 1 tube (15 µg). |
| Ready-to-use - Package B (3 gene specific shRNAs) | 8~11 | 1. With 90% transfection efficiency, at least one shRNA plasmid will achieve 70% mRNA interference. 2. Interference efficiency cannot be guaranteed if the target gene expression is very low. 3. Shipping: 100 µg endotoxin-free plasmid (liquid), 15 µg endotoxin-free negative control plasmid (dry powder), ready for direct use in cell transfections. | Liquid plasmid: 3 tubes (100 µg/tube); Stab culture: 3 tubes; Negative control plasmid: 1 tube (15 µg). |
| Ready-to-use - Package A (4 gene specific shRNAs) | 8~11 | 1. With 90% transfection efficiency, at least one shRNA plasmid will achieve 70% mRNA interference. 2. Interference efficiency cannot be guaranteed if the target gene expression is very low. 3. Shipping: 100 µg endotoxin-free plasmid (liquid), 15 µg endotoxin-free negative control plasmid (dry powder), ready for direct use in cell transfections. | Liquid plasmid: 4 tubes (100 µg/tube); Stab culture: 4 tubes; Negative control plasmid: 1 tube (15 µg). |
| shRNA (NC) | In Stock | The control contains an NC sequence and is not an empty vector. | Lyophilized shRNA control plasmid: 1 tube (15 µg); Stab culture: 1 tube. |
Download the order form right and email it to info@tsingke.com.cn,or "Send Your Request" to submit your inquiry online.
Efficient construction: Rapid generation of shRNA expression plasmids via gene synthesis, eliminating tedious cloning steps.
Professional optimization: Customized design for any gene from any species, with sequence optimization to enhance knockdown efficiency and minimize off-target effects.
Versatile vectors: A wide range of lentiviral backbones available; shRNA can be delivered by direct transfection or packaged into lentiviral particles for infection.
Easy selection: Vectors carry common resistance markers (e.g., PURO/NEO) for stable cell line establishment; fluorescent markers (e.g.,GFP/mcherry) allow real-time monitoring of transfection/infection efficiency and enable flow cytometry sorting.
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