Tsingke offers comprehensive gene cloning services, including PCR cloning and site-directed mutagenesis. Leveraging specialized platforms and extensive expertise, Tsingke ensures accurate, high-efficiency, and reliable cloning solutions for diverse research and engineering applications.
Guaranteed with Sanger sequencing and NGS.
Not limited by restriction enzyme cutting sites, Introduce your mutations at any site.
Clone target gene fragment at any site in any vector system.
All mutations found within a 30-base.
Cost-effective-region will be defined as one whole mutation.
| Type | Introduction |
PCR Cloning&Subcloning | 100% sequence accuracy: Guaranteed with Sanger sequencing and NGS Not limited by restriction enzyme cutting sites Customizable: Clone target gene fragment at any site in any vector system |
| Site-directed Mutagenesis | 100% Sequence Accuracy: Guaranteed with Sanger sequencing and NGS Unlimited Sites: Introduce your mutations at any site Cost-effective: All mutations found within a 30-base region will be defined as one whole mutation |
Target Fragment Preparation:
A.PCR Cloning&Subcloning: PCR amplification or restriction enzyme digestion of the target fragment from the provided plasmid.
B.Site-directed Mutagenesis: Mutant Primer Design & Synthesis&PCR amplification
Target Fragment Preparation:
A.PCR Cloning&Subcloning: PCR amplification or restriction enzyme digestion of the target fragment from the provided plasmid.
B.Site-directed Mutagenesis: Mutant Primer Design & Synthesis&PCR amplification
Ligation: Ligation of the target fragment with the destination vector to form recombinant plasmids.
Ligation: Ligation of the target fragment with the destination vector to form recombinant plasmids.
Screening: Verification of ligation efficiency and construct integrity
Screening: Verification of ligation efficiency and construct integrity
Quality Control: QC for NGS & Sanger sequencing
Quality Control: QC for NGS & Sanger sequencing
Delivery: Shipped as verified positive recombinant plasmid with a detailed quality control report (including screening results, concentration, purity data, and gel images).
Delivery: Shipped as verified positive recombinant plasmid with a detailed quality control report (including screening results, concentration, purity data, and gel images).
Cloning PCR products into the most suitable expression vectors.
Flexibly transferring key genetic elements across different vector platforms to optimize experimental systems.
Download the "Tsingke Gene Synthesis Order Form" from our website, fill in the relevant fields according to your needs, and send it via email to info@tsingke.com.cn to place your order.
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