Gene Delivery

Adeno-associated virus(AAV):Suitable for long-term gene expression and applications with high safety requirements (e.g., gene therapy).

Advantages: Long-term expression, high safety.

Disadvantages: Lower transduction efficiency, small gene capacity.

Lentivirus(LV):Suitable for stable transduction and long-term gene expression (e.g., cell therapy).

Advantages: High transduction efficiency, broad cell type applicability, long-term expression.

Disadvantages: Potential genome integration, safety risks.

Adenovirus (ADV):Suitable for short-term expression and large-scale cell transduction (e.g., vaccine development).

Advantages: High transduction efficiency, suitable for large-scale production.

Disadvantages: Short-term expression, can trigger immune response.

Choose the appropriate vector based on your experimental needs.

Common methods for gene overexpression:

Overexpression plasmids: Introduce the target gene into cells through plasmid transfection for overexpression.

Overexpression lentivirus: Use lentiviral vectors to stably transduce the target gene, suitable for long-term gene expression.

CRISPRa transcriptional activation system: Utilize CRISPR technology to activate the expression of endogenous genes, offering precise regulation.

Each method has its own characteristics, and the choice should be based on the experimental objectives and cell types.

Turnaround time varies by vector type and project scale. Typically, AAV, LV, and AdV vectors can be delivered in as fast as 12–18 business days. Larger-scale or customized projects may take longer.