Our recombinant protein expression services provide researchers and enterprises with reliable, high-quality protein production across E. coli, Bacillus subtilis, yeast, insect, mammalian, and cell-free systems. Tailored strategies ensure optimal expression, solubility, and purity for each protein. From rapid verification to small- and large-scale production, we deliver reproducible results with comprehensive analytical reports. High-throughput and one-day expression options accelerate research timelines, while scalable solutions meet production demands. These proteins are ideal for structural studies, functional assays, drug discovery, and antibody development. With our expertise, clients gain confidence in protein quality, efficiency, and practical application in diverse research and industrial projects.
| Expression System | Service Name | Features | Turnaround Time | Service Code |
| E. coli | Standard Service | One plasmid delivered | 3–5 weeks | Tsingke-P01-1 |
| Guaranteed Service | ≥3 strategies provided to ensure protein expression and purity | 4–6 weeks | Tsingke-P01-2 | |
| High-Throughput Service | ≥50 constructs/batch, up to 200 constructs/batch | 2–3 weeks | Tsingke-P01-3 | |
| Rapid Expression Service | Protein expression & purification in a single day | 1–2 weeks | Tsingke-P01-4 | |
| Expression Verification Service | Validation at 3 temperatures and 2 inducer concentrations; SDS-PAGE and expression report provided | 1 week | Tsingke-P01-5 | |
| Bacillus subtilis | Expression Verification Service | Expression verification; positive clones delivered | 2–4 weeks | Tsingke-P02-1 |
| Small-Scale Expression | Small-scale expression & purification, 100 mL | 3–5 weeks | Tsingke-P02-2 | |
| Scale-Up Expression | Scale-up expression & purification; ≥1 L based on client requirements | 4–7 weeks | Tsingke-P02-3 | |
| Yeast | Expression Verification Service | Expression verification; positive clones delivered | 2–3 weeks | Tsingke-P03-1 |
| Small-Scale Expression | Small-scale expression & purification, 100 mL | 3–4 weeks | Tsingke-P03-2 | |
| Scale-Up Expression | Scale-up expression & purification based on small-scale results | 6–9 weeks | Tsingke-P03-3 | |
| Insect (Baculovirus) | Small-Scale Expression | Small-scale expression & purification, 200 mL | 5–7 weeks | Tsingke-P04-1 |
| Scale-Up Expression | Scale-up expression & purification based on small-scale results | 7–9 weeks | Tsingke-P04-2 | |
| Mammalian Cell | Small-Scale Expression | Small-scale transient expression & purification, 100 mL CHO or HEK293 | 3–5 weeks | Tsingke-P05-1 |
| Scale-Up Expression | Scale-up expression & purification based on small-scale results | 5–7 weeks | Tsingke-P05-2 | |
| Cell-free protein expression (prokaryotic system) | Prokaryotic cell-free expression system | Different strategies for non-membrane and membrane proteins; analysis and design tailored to each specific protein | 3–5 weeks | Tsingke-P06 |
Answer: To begin a project, clients need to provide the DNA sequence of the target protein. Any specific requirements regarding expression system, protein quantity, or purity can also be communicated to help design the optimal expression strategy. Additional services, such as endotoxin removal, protein purification, or QC options, can be requested based on the project needs.
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