Frequently Asked Questions

Polyclonal stable cell line (cell pool): This is obtained by directly selecting cells with drug resistance after transfection. It consists of multiple clones from different sources, with varying integration sites and expression levels of the exogenous gene. It has a short production cycle and low cost, suitable for initial screening and high-expression applications.

Monoclonal stable cell line: This is derived from a cell pool through methods such as limiting dilution, and consists of a single clone of cells with stable integration of the exogenous fragment. All cells have the same integration site, ensuring consistency and stability in expression. It is suitable for high-precision research and biopharmaceutical production. However, it has a longer production cycle and higher cost, and is ideal for cell line experiments requiring subcellular localization, difficult-to-infect cells, and low-expression applications.

Each project includes comprehensive validation such as sequencing results, qPCR data, and optional protein-level verification (e.g., Western blot), ensuring reliable and reproducible results.

Overexpression cell lines are faster and more cost-effective to construct, and they are suitable for studying gene function at the protein level. However, expression may be non-physiological due to strong promoters or random genomic integration. Knock-in cell lines, on the other hand, place the gene under its natural promoter, allowing physiologically relevant expression and more accurate functional studies. The trade-off is that knock-in projects are more complex and typically require longer timelines.