Introduction
GC-rich sequences have always been a stumbling block for molecular biology projects. Their complex secondary structures and high melting temperatures often result in low synthesis efficiency and increased error rates. At Tsingke, we have developed an advanced synthesis platform capable of delivering ultra-low mutation rates (<0.04‰), even for the most challenging GC-rich designs. This article shares how our technology overcomes these difficulties and ensures reliable, high-quality results for global researchers.
GC pairs form three hydrogen bonds, making the DNA more stable and harder to denature during synthesis and amplification. This can lead to:
Incomplete synthesis of the target gene
Secondary structures interfering with enzymatic reactions
Reduced yield and quality of the final product
In research and industry applications, these issues can delay projects and increase costs.
| Optimized Sequence Design | Specialized Synthesis Chemistry | Rigorous Quality Control |
| Our bioinformatics tools analyze and adjust codon usage to reduce problematic secondary structures without altering the encoded protein. | We employ proprietary synthesis chemistry and controlled reaction conditions to improve base incorporation accuracy and minimize errors. | Every gene undergoes multiple verification steps, including Sanger sequencing and NGS analysis, to confirm the absence of unwanted mutations. |
A research team approached us with a 2.4 kb gene containing 75% GC content. Using conventional methods, they had failed multiple times to obtain a complete, correct sequence.
With Tsingke’s optimized synthesis strategy:
Turnaround time: 10 working days
Verified mutation rate: <0.04‰
100% sequence accuracy confirmed by NGS
The result enabled the client to proceed with functional studies without any additional rework.
Use in-silico analysis to predict and minimize secondary structures before ordering synthesis
Consider splitting extremely long GC-rich genes into smaller fragments
Select a synthesis provider with proven experience and QC processes for high-complexity sequences
GC-rich genes no longer need to be a bottleneck in your research. With advanced design algorithms, specialized synthesis chemistry, and comprehensive QC, Tsingke ensures accuracy and reliability, even for the most challenging projects.
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