Name: ProLong Gene Synthesis
Brand: Beijing Tsingke Biotech Co., Ltd.

Highlights of ProLong Gene Synthesis

Tsingke leads in addressing challenges in genetic synthesis, focusing on advancing long-fragment synthesis and assembly technologies. In gene synthesis, where chemical methods limit lengths to 200 nucleotides, we excel in achieving kilobase to megabase-level genes, even entire genomes, through advanced in vitro assembly technologies. With extensive expertise and ongoing innovation, we’ve surpassed genetic synthesis boundaries. Our breakthroughs include mastering yeast assembly for multiple segments and large fragments, enabling precise and efficient one-step assembly of DNA fragments in Saccharomyces cerevisiae. We proficiently produce industrial-scale 50 kb large fragment DNA, demonstrating the capability to deliver fragments up to 200 kb.

Service Details of ProLong Gene Synthesis

Length	Turnaround time (Calendar Day)	Vector	Deliverables
>10kb	Evaluation	pCC1413	(1) 1 tube of lyophilized plasmid DNA (about 1-4 μg/tube); (2) QC files: Sequencing map (.abl file); Target sequence (.seq file); COA Report (elec tronic).

Cases of ProLong Gene Synthesis

Currently, Tsingke has successfully developed the Spore Bacillus One-Step Assembly technology to replace traditional extracellular enzyme ligation methods. This technology allows for the rapid and efficient acquisition of 5 kb DNA sequences, enabling the simultaneous assembly of up to 10 fragments in a single step. This significantly shortens the synthesis cycle, reducing the time required by at least half compared to traditional methods. Additionally, we have optimized the yeast assembly system, greatly enhancing the success rate and transformation efficiency of multi-segment assembly. This optimization enables us to successfully complete the assembly of 200 kb DNA.